Noninvasive Imaging of Human Foveal Capillary Network Using Dual-Conjugate Adaptive Optics

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Noninvasive Imaging of Human Foveal Capillary Network Using Dual-Conjugate Adaptive Optics. / Popovic, Zoran; Knutsson, Per; Thaung, Jorgen; Owner-Petersen, Mette; Sjostrand, Johan.

I: Investigative Ophthalmology & Visual Science, Vol. 52, Nr. 5, 2011, s. 2649-2655.

Forskningsoutput: TidskriftsbidragArtikel i vetenskaplig tidskrift

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Popovic, Zoran ; Knutsson, Per ; Thaung, Jorgen ; Owner-Petersen, Mette ; Sjostrand, Johan. / Noninvasive Imaging of Human Foveal Capillary Network Using Dual-Conjugate Adaptive Optics. I: Investigative Ophthalmology & Visual Science. 2011 ; Vol. 52, Nr. 5. s. 2649-2655.

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TY - JOUR

T1 - Noninvasive Imaging of Human Foveal Capillary Network Using Dual-Conjugate Adaptive Optics

AU - Popovic, Zoran

AU - Knutsson, Per

AU - Thaung, Jorgen

AU - Owner-Petersen, Mette

AU - Sjostrand, Johan

PY - 2011

Y1 - 2011

N2 - PURPOSE. To demonstrate noninvasive imaging of human foveal capillary networks with a high-resolution, wide-field, dual-conjugate adaptive optics (DCAO) imaging instrument. METHODS. The foveal capillary networks of five healthy subjects with no previous history of ocular or neurologic disease or surgery were imaged with a novel high-resolution, wide-field DCAO instrument. The foveal avascular zone (FAZ) in each image was defined using a manual procedure. An automated algorithm based on publicly available and custom-written software was used to identify vessels and extract morphologic FAZ and vessel parameters. Capillary densities were calculated in two annular regions of interest (ROIs) outside the FAZ (500 mu m and 750 mu m outer radius from the foveal center) and in the superior, inferior, temporal, and nasal quadrants within the two ROIs. RESULTS. Mean FAZ area was 0.302 +/- 0.100 mm(2), and mean capillary density (length/area) in the inner ROI was 38.0 +/- 4.0 mm(-1) and 36.4 +/- 4.0 mm(-1) in the outer ROI. The difference in ROI capillary density was not significant. There was no significant difference in quadrant capillary density within the two ROIs or between quadrants irrespective of ROI. CONCLUSIONS. The authors have demonstrated a technique for noninvasive imaging and semiautomated detection and analysis of foveal capillaries. In comparison with other studies, their method yielded lower capillary densities than histology but similar results to the current clinical gold standard, fluorescein angiography. The increased field of view of the DCAO instrument opens up new possibilities for high-resolution noninvasive clinical imaging of foveal capillaries. (Invest Ophthalmol Vis Sci. 2011;52:2649-2655) DOI:10.1167/iovs.10-6054

AB - PURPOSE. To demonstrate noninvasive imaging of human foveal capillary networks with a high-resolution, wide-field, dual-conjugate adaptive optics (DCAO) imaging instrument. METHODS. The foveal capillary networks of five healthy subjects with no previous history of ocular or neurologic disease or surgery were imaged with a novel high-resolution, wide-field DCAO instrument. The foveal avascular zone (FAZ) in each image was defined using a manual procedure. An automated algorithm based on publicly available and custom-written software was used to identify vessels and extract morphologic FAZ and vessel parameters. Capillary densities were calculated in two annular regions of interest (ROIs) outside the FAZ (500 mu m and 750 mu m outer radius from the foveal center) and in the superior, inferior, temporal, and nasal quadrants within the two ROIs. RESULTS. Mean FAZ area was 0.302 +/- 0.100 mm(2), and mean capillary density (length/area) in the inner ROI was 38.0 +/- 4.0 mm(-1) and 36.4 +/- 4.0 mm(-1) in the outer ROI. The difference in ROI capillary density was not significant. There was no significant difference in quadrant capillary density within the two ROIs or between quadrants irrespective of ROI. CONCLUSIONS. The authors have demonstrated a technique for noninvasive imaging and semiautomated detection and analysis of foveal capillaries. In comparison with other studies, their method yielded lower capillary densities than histology but similar results to the current clinical gold standard, fluorescein angiography. The increased field of view of the DCAO instrument opens up new possibilities for high-resolution noninvasive clinical imaging of foveal capillaries. (Invest Ophthalmol Vis Sci. 2011;52:2649-2655) DOI:10.1167/iovs.10-6054

U2 - 10.1167/iovs.10-6054

DO - 10.1167/iovs.10-6054

M3 - Article

VL - 52

SP - 2649

EP - 2655

JO - Investigative Ophthalmology & Visual Science

JF - Investigative Ophthalmology & Visual Science

SN - 1552-5783

IS - 5

ER -