Optimization of temperature and enzyme concentration in the enzymatic saccharification of steam-pretreated willow
Forskningsoutput: Tidskriftsbidrag › Artikel i vetenskaplig tidskrift
The effect of temperature and enzyme concentration on the enzymatic hydrolysis of steam-pretreated willow was investigated to obtain the optimal hydrolysis conditions. The willow was pretreated at 220°C for 10 min according to the results of earlier optimization studies of the steam-pretreatment conditions for high glucose yields. The concentrations of two commercial enzymes, Celluclast 2L and Novozyme 188, were varied in the range 0–20% (based on oven-dry substrate). A maximum in the conversion of cellulose to glucose was obtained using a mixture of 15% Celluclast and 3% Novozyme, corresponding to an activity of 11.4 FPU g−1 substrate. Higher enzyme concentrations did not increase the initial hydrolysis rate nor affect the final glucose yield. The effects of temperature in the range 40°C to 60°C were investigated. The temperature affects both the initial hydrolysis rate and the final glucose yield. The highest glucose yield was obtained at 40°C. The yield decreased with increasing temperature, due to increased enzyme deactivation at higher temperatures, while the initial reaction rate increased with increasing temperatures.
|Enheter & grupper|
Ämnesklassifikation (UKÄ) – OBLIGATORISK
|Tidskrift||Enzyme and Microbial Technology|
|Status||Published - 1990|
|Peer review utförd||Ja|