Optimization of temperature and enzyme concentration in the enzymatic saccharification of steam-pretreated willow

TY - JOUR

T1 - Optimization of temperature and enzyme concentration in the enzymatic saccharification of steam-pretreated willow

AU - Eklund, R

AU - Galbe, Mats

AU - Zacchi, Guido

PY - 1990

Y1 - 1990

N2 - The effect of temperature and enzyme concentration on the enzymatic hydrolysis of steam-pretreated willow was investigated to obtain the optimal hydrolysis conditions. The willow was pretreated at 220°C for 10 min according to the results of earlier optimization studies of the steam-pretreatment conditions for high glucose yields. The concentrations of two commercial enzymes, Celluclast 2L and Novozyme 188, were varied in the range 0–20% (based on oven-dry substrate). A maximum in the conversion of cellulose to glucose was obtained using a mixture of 15% Celluclast and 3% Novozyme, corresponding to an activity of 11.4 FPU g−1 substrate. Higher enzyme concentrations did not increase the initial hydrolysis rate nor affect the final glucose yield. The effects of temperature in the range 40°C to 60°C were investigated. The temperature affects both the initial hydrolysis rate and the final glucose yield. The highest glucose yield was obtained at 40°C. The yield decreased with increasing temperature, due to increased enzyme deactivation at higher temperatures, while the initial reaction rate increased with increasing temperatures.

AB - The effect of temperature and enzyme concentration on the enzymatic hydrolysis of steam-pretreated willow was investigated to obtain the optimal hydrolysis conditions. The willow was pretreated at 220°C for 10 min according to the results of earlier optimization studies of the steam-pretreatment conditions for high glucose yields. The concentrations of two commercial enzymes, Celluclast 2L and Novozyme 188, were varied in the range 0–20% (based on oven-dry substrate). A maximum in the conversion of cellulose to glucose was obtained using a mixture of 15% Celluclast and 3% Novozyme, corresponding to an activity of 11.4 FPU g−1 substrate. Higher enzyme concentrations did not increase the initial hydrolysis rate nor affect the final glucose yield. The effects of temperature in the range 40°C to 60°C were investigated. The temperature affects both the initial hydrolysis rate and the final glucose yield. The highest glucose yield was obtained at 40°C. The yield decreased with increasing temperature, due to increased enzyme deactivation at higher temperatures, while the initial reaction rate increased with increasing temperatures.

U2 - 10.1016/0141-0229(90)90043-P

DO - 10.1016/0141-0229(90)90043-P

M3 - Article

VL - 12

SP - 225

EP - 228

JO - Enzyme and Microbial Technology

JF - Enzyme and Microbial Technology

SN - 0141-0229

IS - 3

ER -