Osmotic pressures of lysozyme solutions from gas-like to crystal states

Forskningsoutput: TidskriftsbidragArtikel i vetenskaplig tidskrift

Abstract

We obtained osmotic pressure data of lysozyme solutions, describing their physical states over a wide concentration range, using osmotic stress for pressures between 0.05 bar and about 40 bar and volume fractions between 0.01 and 0.61. The osmotic pressure vs. volume fraction data consist of a dilute, gas-phase regime, a transition regime with a high-compressibility plateau, and a concentrated regime where the system is nearly incompressible. The first two regimes are shifted towards a higher protein volume fraction upon decreasing the strength or the range of electrostatic interactions. We describe this shift and the overall shape of the experimental data in these two regimes through a model accounting for a steric repulsion, a short-range van der Waals attraction and a screened electrostatic repulsion. The transition is caused by crystallization, as shown by small-angle X-ray scattering. We verified that our data points correspond to thermodynamic equilibria, and thus that they consist of the reference experimental counterpart of a thermodynamic equation of state.

Detaljer

Författare
  • Coralie Pasquier
  • Sylvie Beaufils
  • Antoine Bouchoux
  • Sophie Rigault
  • Bernard Cabane
  • Mikael Lund
  • Valérie Lechevalier
  • Cécile Le Floch-Fouéré
  • Maryvonne Pasco
  • Gilles Pabœuf
  • Javier Pérez
  • Stéphane Pezennec
Enheter & grupper
Externa organisationer
  • University of Rennes I
  • Pierre and Marie Curie University
  • Synchrotron SOLEIL
  • INRA National Institute of Agricultural Research
Forskningsområden

Ämnesklassifikation (UKÄ) – OBLIGATORISK

  • Fysikalisk kemi
Originalspråkengelska
Sidor (från-till)28458-28465
Antal sidor8
TidskriftPhysical Chemistry Chemical Physics
Volym18
Utgivningsnummer41
StatusPublished - 2016
PublikationskategoriForskning
Peer review utfördJa