Overproduction of PIB-Type ATPases.

Forskningsoutput: TidskriftsbidragArtikel i vetenskaplig tidskrift

Abstract

Understanding of the functions and mechanisms of fundamental processes in the cell requires structural information. Structural studies of membrane proteins typically necessitate large amounts of purified and preferably homogenous target protein. Here, we describe a rapid overproduction and purification strategy of a bacterial PIB-type ATPase for isolation of milligrams of target protein per liter Escherichia coli cell culture, with a final quality of the sample which is sufficient for generating high-resolution crystals.

Detaljer

Författare
Enheter & grupper
Forskningsområden

Ämnesklassifikation (UKÄ) – OBLIGATORISK

  • Cell- och molekylärbiologi
Originalspråkengelska
Sidor (från-till)29-36
TidskriftMethods in Molecular Biology
Volym1377
StatusPublished - 2016
PublikationskategoriForskning
Peer review utfördNej