PCR primers for 30 novel gene regions in the nuclear genomes of Lepidoptera

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Abstract

We report primer pairs for 30 new gene regions in the nuclear genomes of Lepidoptera that can be amplified using a standard PCR protocol. The new primers were tested across diverse Lepidoptera, including nonditrysians and a wide selection of ditrysians. These new gene regions give a total of 11,043 bp of DNA sequence data and they show similar variability to traditionally used nuclear gene regions in studies of Lepidoptera. We feel that a PCR-based approach still has its place in molecular systematic studies of Lepidoptera, particularly at the intrafamilial level, and our new set of primers now provides a route to generating phylogenomic datasets using traditional methods.

Detaljer

Författare
Enheter & grupper
Externa organisationer
  • Stockholms universitet
  • University of Turku
Forskningsområden

Ämnesklassifikation (UKÄ) – OBLIGATORISK

  • Zoologi
  • Biokemi och molekylärbiologi
  • Genetik

Nyckelord

Originalspråkengelska
Sidor (från-till)129-141
Antal sidor13
TidskriftZooKeys
Volym2016
Utgivningsnummer596
StatusPublished - 2016
PublikationskategoriForskning
Peer review utfördJa