PCR-mediated deletion of plasmid DNA

Forskningsoutput: TidskriftsbidragLetter

Abstract

The PCR-mediated plasmid DNA deletion method is a simple approach to delete DNA sequences from plasmids using only one round of PCR, with two primers, and without ligation or purification prior to in vivo recombination. By using only PCR, the method is sequence independent and, as shown in this study, is applicable to various sizes of plasmids and deletions using minimal primer design.

Detaljer

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Enheter & grupper
Forskningsområden

Ämnesklassifikation (UKÄ) – OBLIGATORISK

  • Biologiska vetenskaper
Originalspråkengelska
Sidor (från-till)373-375
TidskriftAnalytical Biochemistry
Volym375
Utgåva nummer2
StatusPublished - 2008
PublikationskategoriForskning
Peer review utfördJa