Pedobacter lusitanus sp. nov., isolated from sludge of a deactivated uranium mine

Forskningsoutput: TidskriftsbidragArtikel i vetenskaplig tidskrift

Abstract

Strain NL19T is a Gram-stain-negative, aerobic bacterium that was isolated from sludge of a deactivated uranium mine in Portugal. 16S rRNA gene sequence analysis revealed that strain NL19T is a member of the genus Pedobacter and closely related to the strains Pedobacter himalayensis MTCC 6384T, Pedobacter cryoconitis DSM 14825T, Pedobacter westerhofensis DSM 19036T and Pedobacter hartonius DSM 19033T. It had a DNA G+C content of 40.8 mol%, which agreed with the genus description. The main fatty acids included C16: 1ω7c, C14: 1ω5c, C4: 0, iso-C17: 0, iso-C17: 0 3-OH, C16: 0, anteiso-C15: 0 and iso- C15: 0 3-OH. The main lipids present were phospholipids (60%) and sphingolipids (35%). The most abundant phospholipids included phosphatidylethanolamine, phosphatidylinositol and phosphatidylcholine. Menaquinone-7 (MK-7) was the only isoprenoid quinone detected. DNA–DNA hybridization similarities between strain NL19T and Pedobacter himalayensis MTCC 6384T, Pedobacter cryoconitis DSM 14825T, Pedobacter westerhofensis DSM 19036T and Pedobacter hartonius DSM 19033T were 15.3, 16.2, 11.5 and 16.0%, respectively. Strain NL19T can also be distinguished from these four species based on gyrB and intergenic transcribed spacers (ITS) sequences and by some phenotypic traits such as NaCl tolerance, pH, growth temperature and carbon source utilization. Strain NL19T represents a novel species of the genus Pedobacter, for which the name Pedobacter lusitanus sp. nov. is proposed. The type strain is NL19T (=LMG 29220T=CECT 9028T). An amended description of Pedobacter himalayensis is also included.

Detaljer

Författare
  • Cláudia Covas
  • Tânia Caetano
  • Andreia Cruz
  • Tiago Santos
  • Liliana Dias
  • Guenter Klein
  • Amir Abdulmawjood
  • Luis M. Rodríguez-Alcalá
  • Lígia L. Pimentel
  • Ana Gomes
  • Ana Cristina Freitas
  • Alba Garcia-Serrano
  • Javier Fontecha
  • Sónia Mendo
Externa organisationer
  • University of Aveiro
  • University of Veterinary Medicine Hannover
  • Catholic University of Portugal
  • Bernardo O'Higgins University
  • Autonomous University of Madrid
Forskningsområden

Ämnesklassifikation (UKÄ) – OBLIGATORISK

  • Mikrobiologi

Nyckelord

Originalspråkengelska
Artikelnummer001814
Sidor (från-till)1339-1348
Antal sidor10
TidskriftInternational Journal of Systematic and Evolutionary Microbiology
Volym67
Utgåva nummer5
StatusPublished - 2017 maj 1
PublikationskategoriForskning
Peer review utfördJa
Externt publiceradJa