Perilipin A is essential for the translocation of hormone-sensitive lipase during lipolytic activation

Forskningsoutput: TidskriftsbidragArtikel i vetenskaplig tidskrift

Abstract

A key step in lipolytic activation of adipocytes is the translocation of hormone-sensitive lipase (HSL) from the cytosol to the surface of the lipid storage droplet. Adipocytes from perilipin-null animals have an elevated basal rate of lipolysis compared with adipocytes from Wild-type mice, but fail to respond maximally to lipolytic stimuli. This defect is downstream of the p-adrenergic receptor-adenylyl cyclase complex. Now, we show that HSL is basally associated with lipid droplet surfaces at a low level in pefilipin nulls, but that stimulated translocation from the cytosol to lipid droplets is absent in adipocytes derived from embryonic fibroblasts of perilipin-null mice. We have also reconstructed the HSL translocation reaction in the nonadipocyte Chinese hamster ovary cell line by introduction of GFP-tagged HSL with and without perilipin A. On activation of protein kinase A, HSL-GFP translocates to lipid droplets only in cells that express fully phosphory-latable perilipin A, confirming that perilipin is required to elicit the HSL translocation reaction. Moreover, in Chinese hamster ovary cells that express both HSL and perilipin A, these two proteins cooperate to produce a more rapidly accelerated lipolysis than do cells that express either of these proteins alone, indicating that lipolysis is a concerted reaction mediated by both protein kinase A-phosphorylated HSL and perilipin A.

Detaljer

Författare
  • C Sztalryd
  • GH Xu
  • H Dorward
  • JT Tansey
  • Juan Antonio Contreras
  • AR Kimmel
  • C Londos
Enheter & grupper
Forskningsområden

Ämnesklassifikation (UKÄ) – OBLIGATORISK

  • Medicinska grundvetenskaper

Nyckelord

Originalspråkengelska
Sidor (från-till)1093-1103
TidskriftJournal of Cell Biology
Volym161
Utgåva nummer6
StatusPublished - 2003
PublikationskategoriForskning
Peer review utfördJa