Perilipin and adipophilin expression in lipid loaded macrophages

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Perilipin and adipophilin expression in lipid loaded macrophages. / Persson, Jenny; Degerman, Eva; Nilsson, Jan; Lindholm, Marie.

I: Biochemical and Biophysical Research Communications, Vol. 363, Nr. 4, 2007, s. 1020-1026.

Forskningsoutput: TidskriftsbidragArtikel i vetenskaplig tidskrift

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T1 - Perilipin and adipophilin expression in lipid loaded macrophages

AU - Persson, Jenny

AU - Degerman, Eva

AU - Nilsson, Jan

AU - Lindholm, Marie

PY - 2007

Y1 - 2007

N2 - Lipid-filled macrophages (foam cells) are a defining feature of atherosclerotic plaques. Foam cells contain lipid dro plet- associated proteins that in other cell types regulate lipid turnover. In foam cell such proteins may directly affect lipid droplet formation and lipid efflux. Differentiated primary human monocytes or THP-1 cells were lipid loaded by incubation with aggregated low density lipoproteins (AgLDL) or VLDL resulting in macrophage foam cells with predominantly cholesterol ester or triglyceride-rich lipid droplets, respectively. Lipid droplets were isolated and major proteins identified by mass spectrometry, among them the apolipoprotein B-48 receptor that has not previously been recognized in this context. Expression of two proteins, perilipin and adipophilin, was quantified by Western blots of cell lysates. Perilipin content decreased and adipophilin increased with lipoprotein lipid loading regardless of intracellular neutral lipid composition. This protein expression pattern may hinder lipid turnover in macrophage foam cells, thereby increasing lipid content of atherosclerotic plaques. (C) 2007 Elsevier Inc. All rights reserved.

AB - Lipid-filled macrophages (foam cells) are a defining feature of atherosclerotic plaques. Foam cells contain lipid dro plet- associated proteins that in other cell types regulate lipid turnover. In foam cell such proteins may directly affect lipid droplet formation and lipid efflux. Differentiated primary human monocytes or THP-1 cells were lipid loaded by incubation with aggregated low density lipoproteins (AgLDL) or VLDL resulting in macrophage foam cells with predominantly cholesterol ester or triglyceride-rich lipid droplets, respectively. Lipid droplets were isolated and major proteins identified by mass spectrometry, among them the apolipoprotein B-48 receptor that has not previously been recognized in this context. Expression of two proteins, perilipin and adipophilin, was quantified by Western blots of cell lysates. Perilipin content decreased and adipophilin increased with lipoprotein lipid loading regardless of intracellular neutral lipid composition. This protein expression pattern may hinder lipid turnover in macrophage foam cells, thereby increasing lipid content of atherosclerotic plaques. (C) 2007 Elsevier Inc. All rights reserved.

KW - PAT proteins

KW - lipid droplet

KW - foam cell

KW - macrophage

U2 - 10.1016/j.bbrc.2007.09.074

DO - 10.1016/j.bbrc.2007.09.074

M3 - Article

VL - 363

SP - 1020

EP - 1026

JO - Biochemical and Biophysical Research Communications

T2 - Biochemical and Biophysical Research Communications

JF - Biochemical and Biophysical Research Communications

SN - 1090-2104

IS - 4

ER -