Poly(3-hydroxybutyrate) production by Halomonas boliviensis in fed-batch culture.

Forskningsoutput: TidskriftsbidragArtikel i vetenskaplig tidskrift

Abstract

High poly(3-hydroxybutyrate) (PHB) content and volumetric productivity were achieved by fed-batch culture of Halomonas boliviensis using a defined medium. Initial shake flask cultivations in a minimal medium revealed that the growth of H. boliviensis was supported only when the medium was supplemented with aspartic acid, glycine, or glutamine. Addition of 0.1% (w/v) glutamine in the medium resulted in the highest cell dry weight (CDW; 3.9 g l(-1)). Glutamine was replaced by the less expensive monosodium glutamate (MSG) in the medium without any notable change in the final cell density. Effect of initial concentrations of NH(4)Cl and K(2)HPO(4) on cell growth and PHB accumulation by H. boliviensis was then analyzed using a fed-batch fermentation system. The best conditions for PHB production by H. boliviensis were attained using 0.4% (w/v) NH(4)Cl and 0.22% (w/v) K(2)HPO(4) and adding MSG intermittently to the fermentor. Poly(3-hydroxybutyrate) content and CDW reached 90 wt.% and 23 g l(-1), respectively, after 18 h of cultivation. In order to increase CDW and PHB content, MSG, NH(4)Cl, and K(2)HPO(4) were initially fed to the fermentor to maintain their concentrations at 2%, 0.4%, and 0.22% (w/v), respectively, and subsequently their feed was suppressed. This resulted in a CDW of 44 g l(-1), PHB content of 81 wt.%, and PHB volumetric productivity of 1.1 g l(-1) h(-1).

Detaljer

Författare
  • Jorge Quillaguaman
  • Thuoc Doan Van
  • Hector Guzmán
  • Daniel Guzmán
  • Javier Martín
  • Akaraonye Everest
  • Rajni Hatti-Kaul
Enheter & grupper
Forskningsområden

Ämnesklassifikation (UKÄ) – OBLIGATORISK

  • Industriell bioteknik
Originalspråkengelska
Sidor (från-till)227-232
TidskriftApplied Microbiology and Biotechnology
Volym78
Utgivningsnummer2
StatusPublished - 2008
PublikationskategoriForskning
Peer review utfördJa

Relaterad forskningsoutput

Guzmán, H., 2010, Department of Biotechnology, Lund University. 134 s.

Forskningsoutput: AvhandlingDoktorsavhandling (sammanläggning)

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