Polyelectrolyte complexes as a tool for purification of plasmid DNA background and development

Forskningsoutput: TidskriftsbidragArtikel i vetenskaplig tidskrift

Abstract

The demand for highly purified plasmids in gene therapy and plasmid-based vaccines requires large-scale production of pharmaceutical-grade plasmid. Plasmid DNA was selectively precipitated from a clarified alkaline lysate using the polycation poly(N,N'-dimethyldiallylammonium) chloride which formed insoluble polyelectrolyte complex (PEC) with the plasmid DNA. Soluble PECs of DNA with polycations have earlier been used for cell transformation, but now the focus has been on insoluble PECs. Both DNA and RNA form stable PECs with synthetic polycations. However, it was possible to find a range of salt concentration where plasmid DNA was quantitatively precipitated whereas RNA remained in solution. The precipitated plasmid DNA was resolubilised at high salt concentration and the polycation was removed by gel-filtration. (C) 2004 Elsevier B.V. All rights reserved.

Detaljer

Författare
  • Per-Olof Wahlund
  • Per-Erik Gustavsson
  • Vladimir Izumrudov
  • Per-Olof Larsson
  • Igor Galaev
Enheter & grupper
Forskningsområden

Ämnesklassifikation (UKÄ) – OBLIGATORISK

  • Biokemi och molekylärbiologi
  • Industriell bioteknik

Nyckelord

Originalspråkengelska
Sidor (från-till)121-127
TidskriftJournal of Chromatography. B
Volym807
Utgåva nummer1
StatusPublished - 2004
PublikationskategoriForskning
Peer review utfördJa