Polymerase chain reaction for detection of Mycobacterium tuberculosis

Forskningsoutput: TidskriftsbidragArtikel i vetenskaplig tidskrift

Abstract

A polymerase chain reaction for the specific detection of mycobacteria belonging to the Mycobacterium tuberculosis complex was developed. Using a single primer pair derived from the nucleotide sequence of protein antigen b of M. tuberculosis, we achieved specific amplification of a 419-base-pair DNA fragment in M. tuberculosis and M. bovis. After DNA was extracted from mycobacteria by using a simple, safe lysis procedure, we detected the 419-base-pair sequence in samples containing few mycobacteria. Preliminary data suggested that this technique could be applied to clinical specimens for early and specific diagnosis of tuberculosis.

Detaljer

Författare
  • Ulf Sjöbring
  • Michael Mecklenburg
  • Åse Bengård Andersen
  • Håkan Miörner
Enheter & grupper
Forskningsområden

Ämnesklassifikation (UKÄ) – OBLIGATORISK

  • Mikrobiologi inom det medicinska området
Originalspråkengelska
Sidor (från-till)2200-2204
TidskriftJournal of Clinical Microbiology
Volym28
Utgåva nummer10
StatusPublished - 1990
PublikationskategoriForskning
Peer review utfördJa