Prefractionation of cerebrospinal fluid to enhance glycoprotein concentration prior to structural determination with FT-ICR mass spectrometry

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Abstract

Glycoproteins in cerebrospinal fluid are found to be altered in Alzheimer patients compared to healthy control individuals. We have utilized micro-solution isoelectric focusing and affinity chromatography, prior to gel electrophoresis to enable site-specific structural determination of the N-linked glycans in apolipoprotein J with the use of FT-ICR MS. The albumin depletion method is the most suitable as prefractionation method of CSF prior to 2-DE for structural determination of glycoproteins in the study of neurodegenerative disorders.

Detaljer

Författare
Externa organisationer
  • Göteborgs universitet
Forskningsområden

Nyckelord

Originalspråkengelska
Sidor (från-till)2294-301
Antal sidor8
TidskriftJournal of Proteome Research
Volym4
Utgivningsnummer6
StatusPublished - 2005 dec 13
PublikationskategoriForskning
Peer review utfördJa
Externt publiceradJa