Preparation of a crystallizable mRNA-binding fragment of Moorella thermoacetica elongation factor SelB

Forskningsoutput: TidskriftsbidragArtikel i vetenskaplig tidskrift

Abstract

SelB is a bacterial elongation factor required for the decoding of a UGA stop codon together with a specific mRNA hairpin to selenocysteine. In attempts to crystallize Moorella thermoacetica SelB, a proteolysis process occurred and crystals of a proteolytic fragment were observed. The crystals, which appeared after a year, contained a C-terminal 30 kDa fragment containing the mRNA-binding domain. This fragment was reproduced through recloning. Crystals diffracting to 2.7 Angstrom were obtained.

Detaljer

Författare
  • Maria Selmer
  • R Wilting
  • D Holmlund
  • Xiao-Dong Su
Enheter & grupper
Forskningsområden

Ämnesklassifikation (UKÄ) – OBLIGATORISK

  • Strukturbiologi
Originalspråkengelska
Sidor (från-till)1871-1873
TidskriftActa Crystallographica. Section D: Biological Crystallography
Volym58
StatusPublished - 2002
PublikationskategoriForskning
Peer review utfördJa