Preparative isolation of PCR products using mixed-mode chromatography.

Forskningsoutput: TidskriftsbidragArtikel i vetenskaplig tidskrift

Abstract

The polymerase chain reaction (PCR) has become one of the most useful techniques in molecular biology laboratories around the world. The purification of the target DNA product is, however, often challenging and most users are restricted to employing available commercial kits. The recent developments in mixed-mode chromatography have shown higher selectivity for a variety of nucleic acids containing samples. Capto Adhere is a mixed-mode chromatography resin that offers a high selectivity ligand and is here applied for the purification of amplified DNAs from PCR mixtures in a 10 minutes' single step, with yields above 95%, high linearity and precision for different concentrations.

Detaljer

Författare
  • Tiago Matos
  • Graciela Dos Santos Silva
  • J A Queiroz
  • Leif Bülow
Enheter & grupper
Forskningsområden

Ämnesklassifikation (UKÄ) – OBLIGATORISK

  • Biokemi och molekylärbiologi
Originalspråkengelska
Sidor (från-till)73-75
TidskriftAnalytical Biochemistry
Volym489
Utgåva nummerOnline 14 August 2015
StatusPublished - 2015
PublikationskategoriForskning
Peer review utfördJa