Production and use of recombinant Aβ for aggregation studies

Forskningsoutput: Kapitel i bok/rapport/Conference proceedingKapitel samlingsverk

Abstract

The amyloid β-protein (Aβ) is believed to play a central role in Alzheimer’s disease (AD) pathogenesis and there is great interest in understanding the process of Aβ aggregation, its underlying mechanism and the species generated during aggregation and their biological activity. Although Aβ has been studied for more than 30 years, analysis of its aggregation has been hampered by structural and chemical impurities. Here we provide a detailed protocol for the expression and purification of chemically and structurally homogeneous Aβ monomer. We also describe a method to produce covalent Aβ dimers linked by phenolic coupling of tyrosine residues.

Detaljer

Författare
Enheter & grupper
Externa organisationer
  • Brigham and Women's Hospital / Harvard Medical School
Forskningsområden

Ämnesklassifikation (UKÄ) – OBLIGATORISK

  • Biokemi och molekylärbiologi

Nyckelord

Originalspråkengelska
Titel på värdpublikationMethods in Molecular Biology
FörlagHumana Press
Sidor307-320
Antal sidor14
Volym1777
StatusPublished - 2018 jan 1
PublikationskategoriForskning
Peer review utfördJa

Publikationsserier

NamnMethods in Molecular Biology
Volym1777
ISSN (tryckt)1064-3745