Protein Expression Patterns Associated with Progression of Chronic Obstructive Pulmonary Disease in Bronchoalveolar Lavage of Smokers.

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Protein Expression Patterns Associated with Progression of Chronic Obstructive Pulmonary Disease in Bronchoalveolar Lavage of Smokers. / Plymoth, Amelie; Löfdahl, Claes-Göran; Ekberg-Jansson, Ann; Dahlback, Magnus; Broberg, Per; Foster, Martyn; Fehniger, Thomas E; Marko-Varga, György.

I: Clinical Chemistry, Vol. 53, Nr. 4, 2007, s. 636-644.

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Plymoth, Amelie ; Löfdahl, Claes-Göran ; Ekberg-Jansson, Ann ; Dahlback, Magnus ; Broberg, Per ; Foster, Martyn ; Fehniger, Thomas E ; Marko-Varga, György. / Protein Expression Patterns Associated with Progression of Chronic Obstructive Pulmonary Disease in Bronchoalveolar Lavage of Smokers. I: Clinical Chemistry. 2007 ; Vol. 53, Nr. 4. s. 636-644.

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TY - JOUR

T1 - Protein Expression Patterns Associated with Progression of Chronic Obstructive Pulmonary Disease in Bronchoalveolar Lavage of Smokers.

AU - Plymoth, Amelie

AU - Löfdahl, Claes-Göran

AU - Ekberg-Jansson, Ann

AU - Dahlback, Magnus

AU - Broberg, Per

AU - Foster, Martyn

AU - Fehniger, Thomas E

AU - Marko-Varga, György

N1 - The information about affiliations in this record was updated in December 2015. The record was previously connected to the following departments: Analytical Chemistry (S/LTH) (011001004), Respiratory Medicine and Allergology (013230111)

PY - 2007

Y1 - 2007

N2 - Background: We modeled the expression of proteins in baseline bronchoalveolar lavage (BAL) samples from asymptomatic 60-year-old lifelong current smokers or healthy never-smokers, who were reevaluated after 6 to 7 years to record clinical outcome. Methods: Applying a technology toolbox consisting of replicate 2-dimensional gel separations, image annotation, and mass spectrometry identification, we catalogued a global set of proteins that were differentially expressed in individuals by presence, absence, and intensity scores. Results: By use of multivariate analysis, we selected a subset of proteins that accurately separated smokers from never-smokers based on composite scoring. Follow-up after 6 to 7 years identified a group of individuals who had progressed to chronic obstructive pulmonary disease (COPD), Global Initiative for Chronic Obstructive Lung Disease stage 2. The baseline BAL samples of these eventual COPD patients shared a distinct protein expression profile that could be identified using partial least-squares discriminant analysis. This pattern was not observed in BAL samples of asymptomatic smokers free of COPD at 6- to 7-year follow-up. Conclusions: Our model suggests that certain patterns of protein expression occurring in the airways of long-term smokers may be detected in smokers susceptible to a progression of COPD disease, before disease is clinically evident. (c) 2007 American Association for Clinical Chemistry.

AB - Background: We modeled the expression of proteins in baseline bronchoalveolar lavage (BAL) samples from asymptomatic 60-year-old lifelong current smokers or healthy never-smokers, who were reevaluated after 6 to 7 years to record clinical outcome. Methods: Applying a technology toolbox consisting of replicate 2-dimensional gel separations, image annotation, and mass spectrometry identification, we catalogued a global set of proteins that were differentially expressed in individuals by presence, absence, and intensity scores. Results: By use of multivariate analysis, we selected a subset of proteins that accurately separated smokers from never-smokers based on composite scoring. Follow-up after 6 to 7 years identified a group of individuals who had progressed to chronic obstructive pulmonary disease (COPD), Global Initiative for Chronic Obstructive Lung Disease stage 2. The baseline BAL samples of these eventual COPD patients shared a distinct protein expression profile that could be identified using partial least-squares discriminant analysis. This pattern was not observed in BAL samples of asymptomatic smokers free of COPD at 6- to 7-year follow-up. Conclusions: Our model suggests that certain patterns of protein expression occurring in the airways of long-term smokers may be detected in smokers susceptible to a progression of COPD disease, before disease is clinically evident. (c) 2007 American Association for Clinical Chemistry.

U2 - 10.1373/clinchem.2006.076075

DO - 10.1373/clinchem.2006.076075

M3 - Article

C2 - 17303691

VL - 53

SP - 636

EP - 644

JO - Clinical Chemistry

JF - Clinical Chemistry

SN - 0009-9147

IS - 4

ER -