Protein-Based Capacitive Biosensors: a New Tool for Structure-Activity Relationship Studies

Forskningsoutput: TidskriftsbidragArtikel i vetenskaplig tidskrift

Abstract

The present work reports a new application of a protein-based capacitive biosensor as an in vitro assay for the selectivity study of the bacterial periplasmic protein MerP and four MerP variants. The modified MerP proteins were produced by site-directed mutagenesis of the heavy metal associated motif (HMA). The MerP and modified MerPs selectivity for copper, zinc, cadmium and mercury bivalent ions were investigated and compared. The variations in the proteins affinity were related to the primary structure of the HMA motifs. Key amino acids for copper coordination of metalloproteins that contain the metal binding sequence Gly-Met-Thr-Cys-xxx-xxx-Cys were identified. The results brought insights valid for Menkes and Wilson ATPases. The protein-based capacitive biosensors were a simple and useful tool for studying structure-activity relationships of proteins.

Detaljer

Författare
  • Alessia Mortari
  • Natalhie Campos-Reales
  • Giulia Corda
  • Nigel L. Brown
  • Elisabeth Csöregi
Enheter & grupper
Forskningsområden

Ämnesklassifikation (UKÄ) – OBLIGATORISK

  • Analytisk kemi

Nyckelord

Originalspråkengelska
Sidor (från-till)2677-2684
TidskriftElectroanalysis
Volym20
Utgåva nummer24
StatusPublished - 2008
PublikationskategoriForskning
Peer review utfördJa