Proteome analysis of cerebrospinal fluid proteins in Alzheimer patients

Forskningsoutput: TidskriftsbidragArtikel i vetenskaplig tidskrift

Abstract

By comparing the CSF proteome between Alzheimer disease (AD) patients and controls it may be possible to identify proteins that play a role in the disease process and thus to study the pathogenesis of AD. We used mini-gel technology in a two-dimensional electrophoresis procedure, sensitive SYPRO Ruby staining and mass spectrometry for clinical screening of disease-influenced CSF proteins in 15 AD patients and 12 controls. The levels of six proteins and their isoforms, including proapolipoprotein, apolipoprotein E, beta-2 microglobulin, retinol-binding protein, transthyretin, and ubiquitin, were significantly altered in CSF of AD patients. The most prominently altered proteins were the apolipoproteins, especially proapolipoprotein.

Detaljer

Författare
  • Pia Davidsson
  • Ann Westman-Brinkmalm
  • Carol L Nilsson
  • Maria Lindbjer
  • Linda Paulson
  • Niels Andreasen
  • Magnus Sjögren
  • Kaj Blennow
Externa organisationer
  • Göteborgs universitet
Forskningsområden

Nyckelord

Originalspråkengelska
Sidor (från-till)611-5
Antal sidor5
TidskriftNeuroReport
Volym13
Utgåva nummer5
StatusPublished - 2002 apr 16
PublikationskategoriForskning
Peer review utfördJa
Externt publiceradJa