Proteomic differences between focal and diffuse traumatic brain injury in human brain tissue

Forskningsoutput: TidskriftsbidragArtikel i vetenskaplig tidskrift

Abstract

The early molecular response to severe traumatic brain injury (TBI) was evaluated using biopsies of structurally normal-appearing cortex, obtained at location for intracranial pressure (ICP) monitoring, from 16 severe TBI patients. Mass spectrometry (MS; label free and stable isotope dimethyl labeling) quantitation proteomics showed a strikingly different molecular pattern in TBI in comparison to cortical biopsies from 11 idiopathic normal pressure hydrocephalus patients. Diffuse TBI showed increased expression of peptides related to neurodegeneration (Tau and Fascin, p < 0.05), reduced expression related to antioxidant defense (Glutathione S-transferase Mu 3, Peroxiredoxin-6, Thioredoxin-dependent peroxide reductase; p < 0.05) and increased expression of potential biomarkers (e.g. Neurogranin, Fatty acid-binding protein, heart p < 0.05) compared to focal TBI. Proteomics of human brain biopsies displayed considerable molecular heterogeneity among the different TBI subtypes with consequences for the pathophysiology and development of targeted treatments for TBI.

Detaljer

Författare
  • Sami Abu Hamdeh
  • Ganna Shevchenko
  • Jia Mi
  • Sravani Musunuri
  • Jonas Bergquist
  • Niklas Marklund
Enheter & grupper
Externa organisationer
  • Uppsala universitet, Historiska institutionen
  • Binzhou Medical University
Originalspråkengelska
Artikelnummer6807
Sidor (från-till)1-15
TidskriftScientific Reports
Volym8
Utgivningsnummer1
StatusPublished - 2018 dec 1
PublikationskategoriForskning
Peer review utfördJa