Proteomic study of non-typable Haemophilus influenzae

Forskningsoutput: TidskriftsbidragArtikel i vetenskaplig tidskrift

Abstract

Non-typable Haemophilus influenzae (NTHi) are small, gram-negative bacteria and are strictly human pathogens, causing acute otitis media, sinusitis and community-acquired pneumonia. There is no vaccine available for NTHi, as there is for H. influenzae type b. Recent advances in proteomic techniques are finding novel applications in the field of vaccinology. There are several protein separation techniques available today, each with inherent advantages and disadvantages. We employed a combined proteomics approach, including sequential extraction and analytical two-dimensional polyacrylamide electrophoresis (2D PAGE), and two-dimensional semi-preparative electrophoresis (2D PE), in order to study protein expression in the A4 NTHi strain. Although putative vaccine candidates were identified with both techniques, 11 of 15 proteins identified using the 2D PE approach were not identified by 2D PAGE, demonstrating the complementarily of the two methods.

Detaljer

Författare
  • Kajsa Thorén
  • Elisabet Gustafsson
  • Annica Clevnert
  • Thomas Larsson
  • Jörgen Bergström
  • Carol L Nilsson
Externa organisationer
  • Göteborgs universitet
Forskningsområden

Nyckelord

Originalspråkengelska
Sidor (från-till)219-26
Antal sidor8
TidskriftJournal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences
Volym782
Utgåva nummer1-2
StatusPublished - 2002 dec 25
PublikationskategoriForskning
Peer review utfördJa
Externt publiceradJa