Purification of caveolae by affinity two-phase partitioning using biotinylated antibodies and NeutrAvidin-dextran

Forskningsoutput: TidskriftsbidragArtikel i vetenskaplig tidskrift

Abstract

A new concept for affinity two-phase partitioning was tested. The partitioning was based on the interaction of target membranes with a primary antibody which, in turn, interacted with a biotinylated secondary antibody and NeutrAvidin-dextran in a poly(ethylene glycol)/dextran two-phase system. Caveolae selectively redistributed from the top phase to the NeutrAvidin-dextran-containing bottom phase by employing anti-caveolin as the primary antibody. This immunoaffinity approach was more selective than the established sucrose gradient centrifugation method and resulted in highly purified caveolae from Triton X-100-treated liver and lung plasma membranes. The same approach, employing other selective primary antibodies, should facilitate the purification also of other membrane fractions. (C) 2004 Elsevier Inc. All rights reserved.

Detaljer

Författare
  • Irene Barinaga-Rementeria Ramírez
  • Parisa Abedinpour
  • Bengt Jergil
Enheter & grupper
Forskningsområden

Ämnesklassifikation (UKÄ) – OBLIGATORISK

  • Biologiska vetenskaper

Nyckelord

Originalspråkengelska
Sidor (från-till)17-26
TidskriftAnalytical Biochemistry
Volym331
Utgåva nummer1
StatusPublished - 2004
PublikationskategoriForskning
Peer review utfördJa