Quantitative Label-Free Phosphoproteomics of Six Different Life Stages of the Late Blight Pathogen Phytophthora infestans Reveals Abundant Phosphorylation of Members of the CRN Effector Family

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Quantitative Label-Free Phosphoproteomics of Six Different Life Stages of the Late Blight Pathogen Phytophthora infestans Reveals Abundant Phosphorylation of Members of the CRN Effector Family. / Resjo, Svante; Ali, Ashfaq; Meijer, Harold J. G.; Seidl, Michael F.; Snel, Berend; Sandin, Marianne; Levander, Fredrik; Govers, Francine; Andreasson, Erik.

I: Journal of Proteome Research, Vol. 13, Nr. 4, 2014, s. 1848-1859.

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Resjo, Svante ; Ali, Ashfaq ; Meijer, Harold J. G. ; Seidl, Michael F. ; Snel, Berend ; Sandin, Marianne ; Levander, Fredrik ; Govers, Francine ; Andreasson, Erik. / Quantitative Label-Free Phosphoproteomics of Six Different Life Stages of the Late Blight Pathogen Phytophthora infestans Reveals Abundant Phosphorylation of Members of the CRN Effector Family. I: Journal of Proteome Research. 2014 ; Vol. 13, Nr. 4. s. 1848-1859.

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TY - JOUR

T1 - Quantitative Label-Free Phosphoproteomics of Six Different Life Stages of the Late Blight Pathogen Phytophthora infestans Reveals Abundant Phosphorylation of Members of the CRN Effector Family

AU - Resjo, Svante

AU - Ali, Ashfaq

AU - Meijer, Harold J. G.

AU - Seidl, Michael F.

AU - Snel, Berend

AU - Sandin, Marianne

AU - Levander, Fredrik

AU - Govers, Francine

AU - Andreasson, Erik

PY - 2014

Y1 - 2014

N2 - The oomycete Phytophthora infestans is the causal agent of late blight in potato and tomato. Since the underlying processes that govern pathogenicity and development in P. infestans are largely unknown, we have performed a large-scale phosphoproteomics study of six different P. infestans life stages. We have obtained quantitative data for 2922 phosphopeptides and compared their abundance. Life-stages-pecific phosphopeptides include ATP-binding cassette transporters and a kinase that only occurs in appressoria. In an extended data set, we identified 2179 phosphorylation sites and deduced 22 phosphomotifs. Several of the phosphomotifs matched consensus sequences of kinases that occur in P. infestans but not Arabidopsis. In addition, we detected tyrosine phosphopeptides that are potential targets of kinases resembling mammalian tyrosine kinases. Among the phosphorylated proteins are members of the RXLR and Crinkler effector families. The latter are phosphorylated in several life stages and at multiple positions, in sites that are conserved between different members of the Crinkler family. This indicates that proteins in the Crinkler family have functions beyond their putative role as (necrosis-inducing) effectors. This phosphoproteomics data will be instrumental for studies on oomycetes and host oomycete interactions. The data sets have been deposited to ProteomeXchange (identifier PXD000433).

AB - The oomycete Phytophthora infestans is the causal agent of late blight in potato and tomato. Since the underlying processes that govern pathogenicity and development in P. infestans are largely unknown, we have performed a large-scale phosphoproteomics study of six different P. infestans life stages. We have obtained quantitative data for 2922 phosphopeptides and compared their abundance. Life-stages-pecific phosphopeptides include ATP-binding cassette transporters and a kinase that only occurs in appressoria. In an extended data set, we identified 2179 phosphorylation sites and deduced 22 phosphomotifs. Several of the phosphomotifs matched consensus sequences of kinases that occur in P. infestans but not Arabidopsis. In addition, we detected tyrosine phosphopeptides that are potential targets of kinases resembling mammalian tyrosine kinases. Among the phosphorylated proteins are members of the RXLR and Crinkler effector families. The latter are phosphorylated in several life stages and at multiple positions, in sites that are conserved between different members of the Crinkler family. This indicates that proteins in the Crinkler family have functions beyond their putative role as (necrosis-inducing) effectors. This phosphoproteomics data will be instrumental for studies on oomycetes and host oomycete interactions. The data sets have been deposited to ProteomeXchange (identifier PXD000433).

KW - Phytophthora infestans

KW - late blight

KW - potato

KW - protein phosphorylation

KW - proteomics

KW - phosphoproteomics

KW - effectors

KW - Crinklers

KW - CRN proteins

KW - tyrosine phosphorylation

KW - phosphorylation motifs

KW - appressorium

U2 - 10.1021/pr4009095

DO - 10.1021/pr4009095

M3 - Article

VL - 13

SP - 1848

EP - 1859

JO - Journal of Proteome Research

T2 - Journal of Proteome Research

JF - Journal of Proteome Research

SN - 1535-3893

IS - 4

ER -