Quantitative proteomic analysis identifies proteins and pathways related to neuronal development in differentiated SH-SY5Y neuroblastoma cells

Forskningsoutput: TidskriftsbidragArtikel i vetenskaplig tidskrift

Abstract

SH-SY5Y neuroblastoma cells are susceptible to differentiation using retinoic acid (RA) and brain-derived neurotrophic factor (BDNF), providing a model of neuronal differentiation. We compared SH-SY5Y cells proteome before and after RA/BDNF treatment using iTRAQ and phosphopeptide enrichment strategies. We identified 5587 proteins, 366 of them with differential abundance. Differentiated cells expressed proteins related to neuronal development, and, undifferentiated cells expressed proteins involved in cell proliferation. Interactive network covered focal adhesion, cytoskeleton dynamics and neurodegenerative diseases processes and regulation of mitogen-activated protein kinase-related signaling pathways; key proteins involved in those processes might be explored as markers for neuronal differentiation.

Detaljer

Författare
  • Jimmy Rodriguez Murillo
  • Livia Goto-Silva
  • Aniel Sánchez
  • Fábio C.S. Nogueira
  • Gilberto B. Domont
  • Magno Junqueira
Enheter & grupper
Externa organisationer
  • Federal University of Rio de Janeiro
  • Skåne University Hospital
  • D'Or Institute for Research and Education
Forskningsområden

Ämnesklassifikation (UKÄ) – OBLIGATORISK

  • Cell- och molekylärbiologi
  • Neurovetenskaper

Nyckelord

Originalspråkengelska
Sidor (från-till)1-11
Antal sidor11
TidskriftEuPA Open Proteomics
Volym16
StatusPublished - 2017 sep 1
PublikationskategoriForskning
Peer review utfördJa