Rapid amyloid-β oligomer and protofibril accumulation in traumatic brain injury

Forskningsoutput: TidskriftsbidragArtikel i vetenskaplig tidskrift

Abstract

Deposition of amyloid-β (Aβ) is central to Alzheimer's disease (AD) pathogenesis and associated with progressive neurodegeneration in traumatic brain injury (TBI). We analyzed predisposing factors for Aβ deposition including monomeric Aβ40, Aβ42 and Aβ oligomers/protofibrils, Aβ species with pronounced neurotoxic properties, following human TBI. Highly selective ELISAs were used to analyze N-terminally intact and truncated Aβ40 and Aβ42, as well as Aβ oligomers/protofibrils, in human brain tissue, surgically resected from severe TBI patients (n=12; mean age 49.5±19 years) due to life-threatening brain swelling/hemorrhage within one week post-injury. The TBI tissues were compared to post-mortem AD brains (n=5), to post-mortem tissue of neurologically intact (NI) subjects (n=4) and to cortical biopsies obtained at surgery for idiopathic normal pressure hydrocephalus patients (iNPH; n=4). The levels of Aβ40 and Aβ42 were not elevated by TBI. The levels of Aβ oligomers/protofibrils in TBI were similar to those in the significantly older AD patients and increased compared to NI and iNPH controls (P<0.05). Moreover, TBI patients carrying the AD risk genotype Apolipoprotein E epsilon3/4 (APOE ε3/4; n=4) had increased levels of Aβ oligomers/protofibrils (P<0.05) and of both N-terminally intact and truncated Aβ42 (P<0.05) compared to APOE ε3/4-negative TBI patients (n=8). Neuropathological analysis showed insoluble Aβ aggregates (commonly referred to as Aβ plaques) in three TBI patients, all of whom were APOE ε3/4 carriers. We conclude that soluble intermediary Aβ aggregates form rapidly after TBI, especially among APOE ε3/4 carriers. Further research is needed to determine whether these aggregates aggravate the clinical short- and long-term outcome in TBI.

Detaljer

Författare
  • Sami Abu Hamdeh
  • Erik Rollman Waara
  • Christer Möller
  • Linda Söderberg
  • Hans Basun
  • Irina Alafuzoff
  • Lars Hillered
  • Lars Lannfelt
  • Martin Ingelsson
  • Niklas Marklund
Enheter & grupper
Externa organisationer
  • Uppsala universitet, Historiska institutionen
Forskningsområden

Nyckelord

Originalspråkengelska
Sidor (från-till)451-462
TidskriftBrain Pathology
Volym28
Utgivningsnummer4
Tidigt onlinedatum2017
StatusPublished - 2018 jul
PublikationskategoriForskning
Peer review utfördJa