Rapid test for lung maturity, based on spectroscopy of gastric aspirate, predicted respiratory distress syndrome with high sensitivity

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Rapid test for lung maturity, based on spectroscopy of gastric aspirate, predicted respiratory distress syndrome with high sensitivity. / Verder, Henrik; Heiring, Christian; Clark, Howard; Sweet, David; Jessen, Torben E.; Ebbesen, Finn; Björklund, Lars J.; Andreasson, Bengt; Bender, Lars; Bertelsen, Aksel; Dahl, Marianne; Eschen, Christian; Fenger-Grøn, Jesper; Hoffmann, Stine F.; Höskuldsson, Agnar; Bruusgaard-Mouritsen, Maria; Lundberg, Fredrik; Postle, Anthony D.; Schousboe, Peter; Schmidt, Peter; Stanchev, Hristo; Sørensen, Lars.

I: Acta Paediatrica, International Journal of Paediatrics, Vol. 106, Nr. 3, 2017, s. 430-437.

Forskningsoutput: TidskriftsbidragArtikel i vetenskaplig tidskrift

Harvard

Verder, H, Heiring, C, Clark, H, Sweet, D, Jessen, TE, Ebbesen, F, Björklund, LJ, Andreasson, B, Bender, L, Bertelsen, A, Dahl, M, Eschen, C, Fenger-Grøn, J, Hoffmann, SF, Höskuldsson, A, Bruusgaard-Mouritsen, M, Lundberg, F, Postle, AD, Schousboe, P, Schmidt, P, Stanchev, H & Sørensen, L 2017, 'Rapid test for lung maturity, based on spectroscopy of gastric aspirate, predicted respiratory distress syndrome with high sensitivity', Acta Paediatrica, International Journal of Paediatrics, vol. 106, nr. 3, s. 430-437. https://doi.org/10.1111/apa.13683

APA

CBE

Verder H, Heiring C, Clark H, Sweet D, Jessen TE, Ebbesen F, Björklund LJ, Andreasson B, Bender L, Bertelsen A, Dahl M, Eschen C, Fenger-Grøn J, Hoffmann SF, Höskuldsson A, Bruusgaard-Mouritsen M, Lundberg F, Postle AD, Schousboe P, Schmidt P, Stanchev H, Sørensen L. 2017. Rapid test for lung maturity, based on spectroscopy of gastric aspirate, predicted respiratory distress syndrome with high sensitivity. Acta Paediatrica, International Journal of Paediatrics. 106(3):430-437. https://doi.org/10.1111/apa.13683

MLA

Vancouver

Author

Verder, Henrik ; Heiring, Christian ; Clark, Howard ; Sweet, David ; Jessen, Torben E. ; Ebbesen, Finn ; Björklund, Lars J. ; Andreasson, Bengt ; Bender, Lars ; Bertelsen, Aksel ; Dahl, Marianne ; Eschen, Christian ; Fenger-Grøn, Jesper ; Hoffmann, Stine F. ; Höskuldsson, Agnar ; Bruusgaard-Mouritsen, Maria ; Lundberg, Fredrik ; Postle, Anthony D. ; Schousboe, Peter ; Schmidt, Peter ; Stanchev, Hristo ; Sørensen, Lars. / Rapid test for lung maturity, based on spectroscopy of gastric aspirate, predicted respiratory distress syndrome with high sensitivity. I: Acta Paediatrica, International Journal of Paediatrics. 2017 ; Vol. 106, Nr. 3. s. 430-437.

RIS

TY - JOUR

T1 - Rapid test for lung maturity, based on spectroscopy of gastric aspirate, predicted respiratory distress syndrome with high sensitivity

AU - Verder, Henrik

AU - Heiring, Christian

AU - Clark, Howard

AU - Sweet, David

AU - Jessen, Torben E.

AU - Ebbesen, Finn

AU - Björklund, Lars J.

AU - Andreasson, Bengt

AU - Bender, Lars

AU - Bertelsen, Aksel

AU - Dahl, Marianne

AU - Eschen, Christian

AU - Fenger-Grøn, Jesper

AU - Hoffmann, Stine F.

AU - Höskuldsson, Agnar

AU - Bruusgaard-Mouritsen, Maria

AU - Lundberg, Fredrik

AU - Postle, Anthony D.

AU - Schousboe, Peter

AU - Schmidt, Peter

AU - Stanchev, Hristo

AU - Sørensen, Lars

PY - 2017

Y1 - 2017

N2 - Aim: Respiratory distress syndrome (RDS) is a major cause of mortality and morbidity in premature infants. By the time symptoms appear, it may already be too late to prevent a severe course, with bronchopulmonary dysplasia or mortality. We aimed to develop a rapid test of lung maturity for targeting surfactant supplementation. Methods: Concentrations of the most surface-active lung phospholipid dipalmitoylphosphatidylcholine and sphingomyelin in gastric aspirates from premature infants were measured by mass spectrometry and expressed as the lecithin/sphingomyelin ratio (L/S). The same aspirates were analysed with mid-infrared spectroscopy. Subsequently, L/S was measured in gastric aspirates and oropharyngeal secretions from another group of premature infants using spectroscopy and the results were compared with RDS development. The 10-minute analysis required 10 μL of aspirate. Results: An L/S algorithm was developed based on 89 aspirates. Subsequently, gastric aspirates were sampled in 136 infants of 24-31 weeks of gestation and 61 (45%) developed RDS. The cut-off value of L/S was 2.2, sensitivity was 92%, and specificity was 73%. In 59 cases, the oropharyngeal secretions had less valid L/S than gastric aspirate results. Conclusion: Our rapid test for lung maturity, based on spectroscopy of gastric aspirate, predicted RDS with high sensitivity.

AB - Aim: Respiratory distress syndrome (RDS) is a major cause of mortality and morbidity in premature infants. By the time symptoms appear, it may already be too late to prevent a severe course, with bronchopulmonary dysplasia or mortality. We aimed to develop a rapid test of lung maturity for targeting surfactant supplementation. Methods: Concentrations of the most surface-active lung phospholipid dipalmitoylphosphatidylcholine and sphingomyelin in gastric aspirates from premature infants were measured by mass spectrometry and expressed as the lecithin/sphingomyelin ratio (L/S). The same aspirates were analysed with mid-infrared spectroscopy. Subsequently, L/S was measured in gastric aspirates and oropharyngeal secretions from another group of premature infants using spectroscopy and the results were compared with RDS development. The 10-minute analysis required 10 μL of aspirate. Results: An L/S algorithm was developed based on 89 aspirates. Subsequently, gastric aspirates were sampled in 136 infants of 24-31 weeks of gestation and 61 (45%) developed RDS. The cut-off value of L/S was 2.2, sensitivity was 92%, and specificity was 73%. In 59 cases, the oropharyngeal secretions had less valid L/S than gastric aspirate results. Conclusion: Our rapid test for lung maturity, based on spectroscopy of gastric aspirate, predicted RDS with high sensitivity.

KW - Gastric aspirate

KW - Lung surfactant

KW - Mid-infrared spectroscopy

KW - Prematurity

KW - Respiratory distress syndrome

UR - http://www.scopus.com/inward/record.url?scp=85007374799&partnerID=8YFLogxK

U2 - 10.1111/apa.13683

DO - 10.1111/apa.13683

M3 - Article

VL - 106

SP - 430

EP - 437

JO - Acta Pædiatrica

T2 - Acta Pædiatrica

JF - Acta Pædiatrica

SN - 1651-2227

IS - 3

ER -