Rat stomach ECL cells: mode of activation of histidine decarboxylase

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Rat stomach ECL cells: mode of activation of histidine decarboxylase. / Zhao, CM; Chen, D; Yamada, H; de la Cour, Charlotta; Lindstrom, E; Persson, L; Håkanson, Rolf.

I: Regulatory Peptides, Vol. 114, Nr. 1, 2003, s. 21-27.

Forskningsoutput: TidskriftsbidragArtikel i vetenskaplig tidskrift

Harvard

Zhao, CM, Chen, D, Yamada, H, de la Cour, C, Lindstrom, E, Persson, L & Håkanson, R 2003, 'Rat stomach ECL cells: mode of activation of histidine decarboxylase', Regulatory Peptides, vol. 114, nr. 1, s. 21-27. https://doi.org/10.1016/S0167-0115(03)00063-6

APA

Zhao, CM., Chen, D., Yamada, H., de la Cour, C., Lindstrom, E., Persson, L., & Håkanson, R. (2003). Rat stomach ECL cells: mode of activation of histidine decarboxylase. Regulatory Peptides, 114(1), 21-27. https://doi.org/10.1016/S0167-0115(03)00063-6

CBE

Zhao CM, Chen D, Yamada H, de la Cour C, Lindstrom E, Persson L, Håkanson R. 2003. Rat stomach ECL cells: mode of activation of histidine decarboxylase. Regulatory Peptides. 114(1):21-27. https://doi.org/10.1016/S0167-0115(03)00063-6

MLA

Vancouver

Zhao CM, Chen D, Yamada H, de la Cour C, Lindstrom E, Persson L et al. Rat stomach ECL cells: mode of activation of histidine decarboxylase. Regulatory Peptides. 2003;114(1):21-27. https://doi.org/10.1016/S0167-0115(03)00063-6

Author

Zhao, CM ; Chen, D ; Yamada, H ; de la Cour, Charlotta ; Lindstrom, E ; Persson, L ; Håkanson, Rolf. / Rat stomach ECL cells: mode of activation of histidine decarboxylase. I: Regulatory Peptides. 2003 ; Vol. 114, Nr. 1. s. 21-27.

RIS

TY - JOUR

T1 - Rat stomach ECL cells: mode of activation of histidine decarboxylase

AU - Zhao, CM

AU - Chen, D

AU - Yamada, H

AU - de la Cour, Charlotta

AU - Lindstrom, E

AU - Persson, L

AU - Håkanson, Rolf

PY - 2003

Y1 - 2003

N2 - Histidine decarboxylase (HDC) occurs in ECL cells in the oxyntic mucosa of rat stomach. It is activated by gastrin. Refeeding of fasted rats or treatment with the proton pump inhibitor omeprazole promptly raised the serum gastrin concentration and consequently the HDC activity and the HDC protein content of the oxyntic mucosa. The food- and omeprazole-induced increase in HDC mRNA expression in the oxyntic mucosa was modest by comparison. Blockade of translation (cycloheximide) but not transcription (actinomycin D) prevented the postprandial rise in HDC activity. The half-life of HDC activity (after blockade of translation) was 94 min in omeprazole-treated rats and 55 min in fasted controls. The rate of enzyme synthesis was estimated to be 15 times higher in omeprazole-treated rats than in fasted controls. Inhibition of histamine uptake into ECL-cell granules by reserpine, a blocker of the vesicular monoamine transporter type-2, lowered the HDC activity and prevented the gastrin-induced HDC activation. We suggest that HDC activation reflects enhanced transcription, translation and/or posttranslational enzyme activation as well as stabilization, and that a high cytosolic histamine concentration suppresses HDC activation. (C) 2003 Elsevier Science B.V. All rights reserved.

AB - Histidine decarboxylase (HDC) occurs in ECL cells in the oxyntic mucosa of rat stomach. It is activated by gastrin. Refeeding of fasted rats or treatment with the proton pump inhibitor omeprazole promptly raised the serum gastrin concentration and consequently the HDC activity and the HDC protein content of the oxyntic mucosa. The food- and omeprazole-induced increase in HDC mRNA expression in the oxyntic mucosa was modest by comparison. Blockade of translation (cycloheximide) but not transcription (actinomycin D) prevented the postprandial rise in HDC activity. The half-life of HDC activity (after blockade of translation) was 94 min in omeprazole-treated rats and 55 min in fasted controls. The rate of enzyme synthesis was estimated to be 15 times higher in omeprazole-treated rats than in fasted controls. Inhibition of histamine uptake into ECL-cell granules by reserpine, a blocker of the vesicular monoamine transporter type-2, lowered the HDC activity and prevented the gastrin-induced HDC activation. We suggest that HDC activation reflects enhanced transcription, translation and/or posttranslational enzyme activation as well as stabilization, and that a high cytosolic histamine concentration suppresses HDC activation. (C) 2003 Elsevier Science B.V. All rights reserved.

KW - translation/posttranslation

KW - transcription

KW - rat stomach

KW - protein

KW - HDC

KW - histidine decarboxylase (HDC) activity

KW - ECL cells

KW - histamine

U2 - 10.1016/S0167-0115(03)00063-6

DO - 10.1016/S0167-0115(03)00063-6

M3 - Article

VL - 114

SP - 21

EP - 27

JO - Regulatory Peptides

JF - Regulatory Peptides

SN - 1873-1686

IS - 1

ER -