Real-time PCR-assay in the delivery suite for determination of group B streptococcal colonization in a setting with risk-based antibiotic prophylaxis

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Bibtex

@article{086680481b9644d38a509da4361b3536,
title = "Real-time PCR-assay in the delivery suite for determination of group B streptococcal colonization in a setting with risk-based antibiotic prophylaxis",
abstract = "Objective: Intrapartum antibiotic prophylaxis (IAP) reduces the incidence of neonatal early onset group B streptococcal infections. The present study investigated if an automated PCR-assay, used bedside by the labor ward personnel was manageable and could decrease the use of IAP in a setting with a risk-based IAP strategy. Methods: The study comprises two phases. Phase 1 was a multicenter, randomized, controlled trial. Women with selected risk-factors were allocated either to PCR-IAP (prophylaxis given if positive or indeterminate) or IAP. A vaginal/rectal swab and superficial swabs from the neonate for conventional culture were also obtained. Phase 2 was non-randomized, assessing an improved version of the assay. Results: Phase 1 included 112 women in the PCR-IAP group and 117 in the IAP group. Excluding indeterminate results, the assay showed a sensitivity of 89{\%} and a specificity of 90{\%}. In 44 {\%} of the PCR assays the result was indeterminate. The use of IAP was lower in the PCR group (53 versus 92{\%}). Phase 2 included 94 women. The proportion of indeterminate results was reduced (15{\%}). The GBS colonization rate was 31{\%}. Conclusion: The PCR assay, in the hands of labor ward personnel, can be useful for selection of women to which IAP should be offered.",
keywords = "Antibiotics, delivery, prophylaxis, real-time PCR, S. agalactiae",
author = "Stellan Hakansson and Karin K{\"a}ll{\'e}n and Maria Bullarbo and Per-Ake Holmgren and Katarina Bremme and Asa Larsson and Margareta Norman and Hakan Noren and Catharina Ortmark-Wrede and Karin Pettersson and Sissel Saltvedt and Birgitta Sondell and Magdalena Tokarska and {von Vultee}, Anna and Bo Jacobsson",
year = "2014",
doi = "10.3109/14767058.2013.818128",
language = "English",
volume = "27",
pages = "328--332",
journal = "Journal of Maternal-Fetal & Neonatal Medicine",
issn = "1476-7058",
publisher = "Taylor & Francis",
number = "4",

}