Recent advances in the characterization of HIV-1 neutralization assays for standardized evaluation of the antibody response to infection and vaccination

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Recent advances in the characterization of HIV-1 neutralization assays for standardized evaluation of the antibody response to infection and vaccination. / Polonis, Victoria R; Brown, Bruce K; Borges, Andrew Rosa; Zolla-Pazner, Susan; Dimitrov, Dimiter S; Zhang, Mei-Yun; Barnett, Susan W; Ruprecht, Ruth M; Scarlatti, Gabriella; Fenyö, Eva Maria; Montefiori, David C; McCutchan, Francine E; Michael, Nelson L.

I: Virology, Vol. 375, Nr. 2, 2008, s. 315-320.

Forskningsoutput: TidskriftsbidragÖversiktsartikel

Harvard

Polonis, VR, Brown, BK, Borges, AR, Zolla-Pazner, S, Dimitrov, DS, Zhang, M-Y, Barnett, SW, Ruprecht, RM, Scarlatti, G, Fenyö, EM, Montefiori, DC, McCutchan, FE & Michael, NL 2008, 'Recent advances in the characterization of HIV-1 neutralization assays for standardized evaluation of the antibody response to infection and vaccination', Virology, vol. 375, nr. 2, s. 315-320. https://doi.org/10.1016/j.virol.2008.02.007

APA

Polonis, V. R., Brown, B. K., Borges, A. R., Zolla-Pazner, S., Dimitrov, D. S., Zhang, M-Y., Barnett, S. W., Ruprecht, R. M., Scarlatti, G., Fenyö, E. M., Montefiori, D. C., McCutchan, F. E., & Michael, N. L. (2008). Recent advances in the characterization of HIV-1 neutralization assays for standardized evaluation of the antibody response to infection and vaccination. Virology, 375(2), 315-320. https://doi.org/10.1016/j.virol.2008.02.007

CBE

Polonis VR, Brown BK, Borges AR, Zolla-Pazner S, Dimitrov DS, Zhang M-Y, Barnett SW, Ruprecht RM, Scarlatti G, Fenyö EM, Montefiori DC, McCutchan FE, Michael NL. 2008. Recent advances in the characterization of HIV-1 neutralization assays for standardized evaluation of the antibody response to infection and vaccination. Virology. 375(2):315-320. https://doi.org/10.1016/j.virol.2008.02.007

MLA

Vancouver

Author

Polonis, Victoria R ; Brown, Bruce K ; Borges, Andrew Rosa ; Zolla-Pazner, Susan ; Dimitrov, Dimiter S ; Zhang, Mei-Yun ; Barnett, Susan W ; Ruprecht, Ruth M ; Scarlatti, Gabriella ; Fenyö, Eva Maria ; Montefiori, David C ; McCutchan, Francine E ; Michael, Nelson L. / Recent advances in the characterization of HIV-1 neutralization assays for standardized evaluation of the antibody response to infection and vaccination. I: Virology. 2008 ; Vol. 375, Nr. 2. s. 315-320.

RIS

TY - JOUR

T1 - Recent advances in the characterization of HIV-1 neutralization assays for standardized evaluation of the antibody response to infection and vaccination

AU - Polonis, Victoria R

AU - Brown, Bruce K

AU - Borges, Andrew Rosa

AU - Zolla-Pazner, Susan

AU - Dimitrov, Dimiter S

AU - Zhang, Mei-Yun

AU - Barnett, Susan W

AU - Ruprecht, Ruth M

AU - Scarlatti, Gabriella

AU - Fenyö, Eva Maria

AU - Montefiori, David C

AU - McCutchan, Francine E

AU - Michael, Nelson L

PY - 2008

Y1 - 2008

N2 - In AIDS vaccine development the pendulum has swung towards a renewed emphasis on the potential role for neutralizing antibodies in a successful global vaccine. It is recognized that vaccine-induced antibody performance, as assessed in the available neutralization assays, may well serve as a "gatekeeper" for HIV-1 subunit vaccine prioritization and advancement. As a result, development of a standardized platform for reproducible measurement of neutralizing antibodies has received considerable attention. Here we review current advancements in our knowledge of the performance of different types of antibodies in a traditional primary cell neutralization assay and the newer, more standardized TZM-bl reporter cell line assay. In light of recently revealed differences (see accompanying article) in the results obtained in these two neutralization formats, parallel evaluation with both platforms should be contemplated as an interim solution until a better understanding of immune correlates of protection is achieved.

AB - In AIDS vaccine development the pendulum has swung towards a renewed emphasis on the potential role for neutralizing antibodies in a successful global vaccine. It is recognized that vaccine-induced antibody performance, as assessed in the available neutralization assays, may well serve as a "gatekeeper" for HIV-1 subunit vaccine prioritization and advancement. As a result, development of a standardized platform for reproducible measurement of neutralizing antibodies has received considerable attention. Here we review current advancements in our knowledge of the performance of different types of antibodies in a traditional primary cell neutralization assay and the newer, more standardized TZM-bl reporter cell line assay. In light of recently revealed differences (see accompanying article) in the results obtained in these two neutralization formats, parallel evaluation with both platforms should be contemplated as an interim solution until a better understanding of immune correlates of protection is achieved.

KW - vaccine immune assessment

KW - standardization

KW - pseudovirus

KW - assays

KW - neutralization

KW - human immunodeficiency virus type I (HIV-1)

KW - antibodies

U2 - 10.1016/j.virol.2008.02.007

DO - 10.1016/j.virol.2008.02.007

M3 - Review article

C2 - 18367229

VL - 375

SP - 315

EP - 320

JO - Virology

JF - Virology

SN - 1096-0341

IS - 2

ER -