Redox balance and carbonylated proteins in limb and heart muscles of cachectic rats

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Redox balance and carbonylated proteins in limb and heart muscles of cachectic rats. / Marin-Corral, Judith; Fontes , Cibely; Pascual-Guardia, Sergi; Sanchez, Francisco; Olivan, Mireia; Argilés, Josep M; Busquets, Sílvia; López-Soriano, Francisco J; Barreiro, Esther.

I: Antioxidants and Redox Signaling, Vol. 12, Nr. 3, 03.2010, s. 365-80.

Forskningsoutput: TidskriftsbidragArtikel i vetenskaplig tidskrift

Harvard

Marin-Corral, J, Fontes , C, Pascual-Guardia, S, Sanchez, F, Olivan, M, Argilés, JM, Busquets, S, López-Soriano, FJ & Barreiro, E 2010, 'Redox balance and carbonylated proteins in limb and heart muscles of cachectic rats', Antioxidants and Redox Signaling, vol. 12, nr. 3, s. 365-80. https://doi.org/10.1089/ars.2009.2818

APA

Marin-Corral, J., Fontes , C., Pascual-Guardia, S., Sanchez, F., Olivan, M., Argilés, J. M., Busquets, S., López-Soriano, F. J., & Barreiro, E. (2010). Redox balance and carbonylated proteins in limb and heart muscles of cachectic rats. Antioxidants and Redox Signaling, 12(3), 365-80. https://doi.org/10.1089/ars.2009.2818

CBE

Marin-Corral J, Fontes C, Pascual-Guardia S, Sanchez F, Olivan M, Argilés JM, Busquets S, López-Soriano FJ, Barreiro E. 2010. Redox balance and carbonylated proteins in limb and heart muscles of cachectic rats. Antioxidants and Redox Signaling. 12(3):365-80. https://doi.org/10.1089/ars.2009.2818

MLA

Vancouver

Marin-Corral J, Fontes C, Pascual-Guardia S, Sanchez F, Olivan M, Argilés JM et al. Redox balance and carbonylated proteins in limb and heart muscles of cachectic rats. Antioxidants and Redox Signaling. 2010 mar;12(3):365-80. https://doi.org/10.1089/ars.2009.2818

Author

Marin-Corral, Judith ; Fontes , Cibely ; Pascual-Guardia, Sergi ; Sanchez, Francisco ; Olivan, Mireia ; Argilés, Josep M ; Busquets, Sílvia ; López-Soriano, Francisco J ; Barreiro, Esther. / Redox balance and carbonylated proteins in limb and heart muscles of cachectic rats. I: Antioxidants and Redox Signaling. 2010 ; Vol. 12, Nr. 3. s. 365-80.

RIS

TY - JOUR

T1 - Redox balance and carbonylated proteins in limb and heart muscles of cachectic rats

AU - Marin-Corral, Judith

AU - Fontes , Cibely

AU - Pascual-Guardia, Sergi

AU - Sanchez, Francisco

AU - Olivan, Mireia

AU - Argilés, Josep M

AU - Busquets, Sílvia

AU - López-Soriano, Francisco J

AU - Barreiro, Esther

PY - 2010/3

Y1 - 2010/3

N2 - In fast- and slow-twitch limb and heart muscles of cachectic rats, redox balance and muscle structure were explored. The nature of the oxidatively modified proteins also was identified in these muscles. Reactive carbonyls, hydroxynonenal (HNE)- and malondialdehyde (MDA)-protein adducts, and antioxidant enzyme levels were determined in limb and heart muscles of cachectic (7 days after inoculation of Yoshida AH-130 ascites hepatoma) and control rats. Moreover, carbonylated proteins were identified (proteomics), and fiber-type composition evaluated (morphometry) in these muscles. In cachectic rats, compared with the controls: (a) HNE- and MDA-protein adducts levels were greater in gastrocnemius, tibialis anterior, soleus, and heart; (b) in the gastrocnemius, type II fiber size was reduced, and the intensity of carbonylated protein immunostaining was significantly greater in these fibers; and (c) proteins involved in glycolysis, ATP production and distribution, carbon dioxide hydration, muscle contraction, and mitochondrial metabolism were significantly more carbonylated in limb and heart muscles. Cancer cachexia alters redox balance in fast- and slow-twitch limb and heart muscles of rats, inducing increased oxidative modifications of key proteins involved in muscle structure and function. Additionally, it induces a reduction in type II fiber size in the gastrocnemius, which is associated with increased protein oxidation.

AB - In fast- and slow-twitch limb and heart muscles of cachectic rats, redox balance and muscle structure were explored. The nature of the oxidatively modified proteins also was identified in these muscles. Reactive carbonyls, hydroxynonenal (HNE)- and malondialdehyde (MDA)-protein adducts, and antioxidant enzyme levels were determined in limb and heart muscles of cachectic (7 days after inoculation of Yoshida AH-130 ascites hepatoma) and control rats. Moreover, carbonylated proteins were identified (proteomics), and fiber-type composition evaluated (morphometry) in these muscles. In cachectic rats, compared with the controls: (a) HNE- and MDA-protein adducts levels were greater in gastrocnemius, tibialis anterior, soleus, and heart; (b) in the gastrocnemius, type II fiber size was reduced, and the intensity of carbonylated protein immunostaining was significantly greater in these fibers; and (c) proteins involved in glycolysis, ATP production and distribution, carbon dioxide hydration, muscle contraction, and mitochondrial metabolism were significantly more carbonylated in limb and heart muscles. Cancer cachexia alters redox balance in fast- and slow-twitch limb and heart muscles of rats, inducing increased oxidative modifications of key proteins involved in muscle structure and function. Additionally, it induces a reduction in type II fiber size in the gastrocnemius, which is associated with increased protein oxidation.

KW - Animals

KW - Cachexia

KW - Electrophoresis

KW - Electrophoresis, Gel, Two-Dimensional

KW - Immunoblotting

KW - Immunohistochemistry

KW - Male

KW - Malondialdehyde

KW - Muscle, Skeletal

KW - Myocardium

KW - Oxidation-Reduction

KW - Protein Carbonylation

KW - Rats

KW - Rats, Wistar

KW - Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization

KW - Journal Article

KW - Research Support, Non-U.S. Gov't

U2 - 10.1089/ars.2009.2818

DO - 10.1089/ars.2009.2818

M3 - Article

C2 - 19737087

VL - 12

SP - 365

EP - 380

JO - Antioxidants and Redox Signaling

JF - Antioxidants and Redox Signaling

SN - 1557-7716

IS - 3

ER -