Regulation of bone sialoprotein mRNA by steroid hormones.

Forskningsoutput: TidskriftsbidragArtikel i vetenskaplig tidskrift

Abstract

In this report we demonstrate an increase in the steady-state level of bone sialoprotein (BSP) mRNA in rat calvaria and a rat osteosarcoma cell line (ROS 17/2.8) after treatment with the synthetic glucocorticoid, dexamethasone. In contrast, 1.25-dihydroxyvitamin D3 reduced the amount of BSP mRNA in calvaria and inhibited the dexamethasone induction in ROS 17/2.8 cells. The increase in BSP mRNA is most likely due to an increase in the transcriptional rate. The stability of mRNA was unchanged after dexamethasone treatment with a half-life of approximately 5 h. Nuclear transcription experiments with nuclei isolated from ROS 17/2.8 cells showed an increased BSP mRNA synthesis in cells treated with dexamethasone.

Detaljer

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Enheter & grupper
Forskningsområden

Ämnesklassifikation (UKÄ) – OBLIGATORISK

  • Oto-rino-laryngologi

Nyckelord

Originalspråkengelska
Sidor (från-till)3183-3186
TidskriftJournal of Cell Biology
Volym109
Utgåva nummer6 Pt 1
StatusPublished - 1989
PublikationskategoriForskning
Peer review utfördJa