Reindeer beta-lactoglobulin crystal structure with pseudo-body-centred noncrystallographic symmetry

Forskningsoutput: TidskriftsbidragArtikel i vetenskaplig tidskrift

Abstract

beta-lactoglobulin (beta LG) belongs to the lipocalin superfamily. Its DNA and protein sequences have been determined and showed that it had nine residue changes from bovine beta LG. Reindeer beta LG, the structure of which was finally determined at 2.1 angstrom resolution in space group P1, crystallized in a unit cell that is both P2-like and P2(1)-like owing to the presence of an almost perfect (but noncrystallographic) body-centring vector. The non-body-centred data could only be observed using a very bright synchrotron beam and a novel refinement strategy was adopted to enable us to use the weak h + k + l = 2n + 1 reflections.

Detaljer

Författare
  • Esko Oksanen
  • Veli-Pekka Jaakola
  • Tiina Tolonen
  • Kaija Valkonen
  • Bo Åkerström
  • Nisse Kalkkinen
  • Vesa Virtanen
  • Adrian Goldman
Enheter & grupper
Forskningsområden

Ämnesklassifikation (UKÄ) – OBLIGATORISK

  • Strukturbiologi
Originalspråkengelska
Sidor (från-till)1369-1374
TidskriftActa Crystallographica. Section D: Biological Crystallography
Volym62
Utgåva nummer11
StatusPublished - 2006
PublikationskategoriForskning
Peer review utfördJa