Relationship between HMF intake and SMF formation in vivo: An animal and human study

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Relationship between HMF intake and SMF formation in vivo : An animal and human study. / Pastoriza de la Cueva, Silvia; Álvarez, Juana; Végvári, Ákos; Montilla-Gómez, Javier; Cruz-López, Olga; Delgado-Andrade, Cristina; Rufián-Henares, José A.

I: Molecular Nutrition and Food Research, Vol. 61, Nr. 3, 1600773, 03.2017.

Forskningsoutput: TidskriftsbidragArtikel i vetenskaplig tidskrift

Harvard

Pastoriza de la Cueva, S, Álvarez, J, Végvári, Á, Montilla-Gómez, J, Cruz-López, O, Delgado-Andrade, C & Rufián-Henares, JA 2017, 'Relationship between HMF intake and SMF formation in vivo: An animal and human study', Molecular Nutrition and Food Research, vol. 61, nr. 3, 1600773. https://doi.org/10.1002/mnfr.201600773

APA

Pastoriza de la Cueva, S., Álvarez, J., Végvári, Á., Montilla-Gómez, J., Cruz-López, O., Delgado-Andrade, C., & Rufián-Henares, J. A. (2017). Relationship between HMF intake and SMF formation in vivo: An animal and human study. Molecular Nutrition and Food Research, 61(3), [1600773]. https://doi.org/10.1002/mnfr.201600773

CBE

Pastoriza de la Cueva S, Álvarez J, Végvári Á, Montilla-Gómez J, Cruz-López O, Delgado-Andrade C, Rufián-Henares JA. 2017. Relationship between HMF intake and SMF formation in vivo: An animal and human study. Molecular Nutrition and Food Research. 61(3). https://doi.org/10.1002/mnfr.201600773

MLA

Vancouver

Pastoriza de la Cueva S, Álvarez J, Végvári Á, Montilla-Gómez J, Cruz-López O, Delgado-Andrade C et al. Relationship between HMF intake and SMF formation in vivo: An animal and human study. Molecular Nutrition and Food Research. 2017 mar;61(3). 1600773. https://doi.org/10.1002/mnfr.201600773

Author

Pastoriza de la Cueva, Silvia ; Álvarez, Juana ; Végvári, Ákos ; Montilla-Gómez, Javier ; Cruz-López, Olga ; Delgado-Andrade, Cristina ; Rufián-Henares, José A. / Relationship between HMF intake and SMF formation in vivo : An animal and human study. I: Molecular Nutrition and Food Research. 2017 ; Vol. 61, Nr. 3.

RIS

TY - JOUR

T1 - Relationship between HMF intake and SMF formation in vivo

T2 - Molecular Nutrition and Food Research

AU - Pastoriza de la Cueva, Silvia

AU - Álvarez, Juana

AU - Végvári, Ákos

AU - Montilla-Gómez, Javier

AU - Cruz-López, Olga

AU - Delgado-Andrade, Cristina

AU - Rufián-Henares, José A.

PY - 2017/3

Y1 - 2017/3

N2 - Scope: 5-Hydroxymethylfurfural (HMF) is a furanic compound produced in heat-processed foods by nonenzymatic browning reactions. HMF has been demonstrated to be hepato- and nephrotoxic in animals with a link to its metabolite 5-sulfooxymethylfurfural (SMF). To date little is known about either the formation of SMF from ingested HMF or the formation of DNA adducts in animals or human beings. Methods and results: To assess SMF in vivo formation, we first performed a study in mice treated with high/low doses of oral HMF. We found increased concentrations of SMF in plasma and DNA SMF-adducts in leukocytes, hepatic tissue, and kidneys by means of LC-MS/MS, but no spatial formation in such tissues was observed by MALDI-MS imaging technology due to low sensitivity. In a second experiment, we measured the exposure to HMF in a Spanish preadolescent population. We analyzed the concentration of HMF metabolites (plasma, urine) and measured, for the first time, the presence of SMF in plasma and DNA SMF-adducts in leukocytes. Conclusion: This study provides the first evidence that oral HMF is readily transformed into SMF in vivo, giving rise to the formation of DNA adducts in a direct relation with HMF intake, both in animals and human beings.

AB - Scope: 5-Hydroxymethylfurfural (HMF) is a furanic compound produced in heat-processed foods by nonenzymatic browning reactions. HMF has been demonstrated to be hepato- and nephrotoxic in animals with a link to its metabolite 5-sulfooxymethylfurfural (SMF). To date little is known about either the formation of SMF from ingested HMF or the formation of DNA adducts in animals or human beings. Methods and results: To assess SMF in vivo formation, we first performed a study in mice treated with high/low doses of oral HMF. We found increased concentrations of SMF in plasma and DNA SMF-adducts in leukocytes, hepatic tissue, and kidneys by means of LC-MS/MS, but no spatial formation in such tissues was observed by MALDI-MS imaging technology due to low sensitivity. In a second experiment, we measured the exposure to HMF in a Spanish preadolescent population. We analyzed the concentration of HMF metabolites (plasma, urine) and measured, for the first time, the presence of SMF in plasma and DNA SMF-adducts in leukocytes. Conclusion: This study provides the first evidence that oral HMF is readily transformed into SMF in vivo, giving rise to the formation of DNA adducts in a direct relation with HMF intake, both in animals and human beings.

KW - 5-Hydroxymethylfurfural

KW - 5-Sulfooxymethylfurfural

KW - Estimated intake

KW - Heat processing

KW - Maillard reaction

UR - http://www.scopus.com/inward/record.url?scp=85009230929&partnerID=8YFLogxK

U2 - 10.1002/mnfr.201600773

DO - 10.1002/mnfr.201600773

M3 - Article

VL - 61

JO - Molecular Nutrition and Food Research

JF - Molecular Nutrition and Food Research

SN - 1613-4133

IS - 3

M1 - 1600773

ER -