Secretory leukocyte protease inhibitor in punch biopsies from human colonic mucosa

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Secretory leukocyte protease inhibitor in punch biopsies from human colonic mucosa. / Nyström, Max; Westin, Ulla Peterson; Linder, Carina; Ohlsson, Kjell.

I: Mediators of Inflammation, Vol. 10, Nr. 5, 2001, s. 269-272.

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Nyström, Max ; Westin, Ulla Peterson ; Linder, Carina ; Ohlsson, Kjell. / Secretory leukocyte protease inhibitor in punch biopsies from human colonic mucosa. I: Mediators of Inflammation. 2001 ; Vol. 10, Nr. 5. s. 269-272.

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TY - JOUR

T1 - Secretory leukocyte protease inhibitor in punch biopsies from human colonic mucosa

AU - Nyström, Max

AU - Westin, Ulla Peterson

AU - Linder, Carina

AU - Ohlsson, Kjell

PY - 2001

Y1 - 2001

N2 - Secretory leukocyte protease inhibitor (SLPI) is a well-known protease inhibitor. Its function is thought to be protease/protease-inhibitor balance. Free proteolytic activity, mainly pancreatic elastase, anionic trypsin and granulocytic elastase, has been demonstrated in faecal extracts from patients with ulcerative colitis. We wanted to verify that SLPI is actually secreted from normal human colonic mucosa. Also, we wanted to ascertain whether studies of SLPI secretion based on punch biopsies were dependent on biopsy area or on biopsy circumference. Normal colonic mucosa was sampled during surgery for colonic cancer. A total of 36 samples from four patients were used. Mucosa preparation was carried out using a punch biopsy technique, and samples of 3, 4 and 6 mm diameter were used. All media contained SLPI at varying concentrations. When expressed in terms of the sample area, the secretion per millimetre-squared seemed to decrease with increasing area. When calculated as secretion per circumference, secretion seemed to be constant. In conclusion, SLPI was secreted from normal human colonic mucosa. The SLPI secretion seemed dependent on the circumference of the biopsy rather than on the area of the biopsy.

AB - Secretory leukocyte protease inhibitor (SLPI) is a well-known protease inhibitor. Its function is thought to be protease/protease-inhibitor balance. Free proteolytic activity, mainly pancreatic elastase, anionic trypsin and granulocytic elastase, has been demonstrated in faecal extracts from patients with ulcerative colitis. We wanted to verify that SLPI is actually secreted from normal human colonic mucosa. Also, we wanted to ascertain whether studies of SLPI secretion based on punch biopsies were dependent on biopsy area or on biopsy circumference. Normal colonic mucosa was sampled during surgery for colonic cancer. A total of 36 samples from four patients were used. Mucosa preparation was carried out using a punch biopsy technique, and samples of 3, 4 and 6 mm diameter were used. All media contained SLPI at varying concentrations. When expressed in terms of the sample area, the secretion per millimetre-squared seemed to decrease with increasing area. When calculated as secretion per circumference, secretion seemed to be constant. In conclusion, SLPI was secreted from normal human colonic mucosa. The SLPI secretion seemed dependent on the circumference of the biopsy rather than on the area of the biopsy.

KW - Anti-leukoprotease

KW - Human cell culture

KW - Punch biopsy

KW - Trauma

UR - http://www.scopus.com/inward/record.url?scp=0035186271&partnerID=8YFLogxK

U2 - 10.1080/09629350120093740

DO - 10.1080/09629350120093740

M3 - Article

VL - 10

SP - 269

EP - 272

JO - Mediators of Inflammation

T2 - Mediators of Inflammation

JF - Mediators of Inflammation

SN - 0962-9351

IS - 5

ER -