Selective immunolesion of cholinergic neurons leads to long-term changes in 5-HT2A receptor levels in hippocampus and frontal cortex.

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Bibtex

@article{b56423ecc87e4e64852c5bde9806c212,
title = "Selective immunolesion of cholinergic neurons leads to long-term changes in 5-HT2A receptor levels in hippocampus and frontal cortex.",
abstract = "Although loss of cholinergic neurons in the basal forebrain is considered a key initial feature in Alzheimer's disease (AD), changes in other transmitter systems, including serotonin and 5-HT2A receptors, are also associated with early AD. The aim of this study was to investigate whether elimination of the cholinergic neurons in the basal forebrain directly affects 5-HT2A receptor levels. For this purpose intraventricular injection of the selective immunotoxin 192 IgG-Saporin was given to rats in doses of either 2.5 or 5 μg. The rats were sacrificed after 1, 2, 4 and 20 weeks. 5-HT2A protein levels were determined by western techniques in frontal cortex and hippocampus. A significant 70% downregulation in frontal cortex and a 100% upregulation in hippocampus of 5-HT2A receptor levels were observed 20 weeks after the cholinergic lesion when using the highest dose of 192 IgG-Saporin. Our results show that cholinergic deafferentation leads to decreased frontal cortex and increased hippoca",
author = "Maurizio Severino and AF Pedersen and V Trajkovska and E Christensen and R Lohals and LM Veng and GM Knudsen and S Aznar",
year = "2007",
doi = "10.1016/j.neulet.2007.09.026",
language = "English",
volume = "428",
pages = "47--51",
journal = "Neuroscience Letters",
issn = "0304-3940",
publisher = "Elsevier",
number = "1",

}