Selective screening of secretory vesicle-associated proteins for autoantigens in type 1 diabetes: VAMP2 and NPY are new minor autoantigens

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Bibtex

@article{0553cb5ff2bb40ca9421032dd731a8cc,
title = "Selective screening of secretory vesicle-associated proteins for autoantigens in type 1 diabetes: VAMP2 and NPY are new minor autoantigens",
abstract = "The four major autoantigens (IA-2, IA-2 beta, GAD65 and insulin) of type 1 diabetes are all associated with dense core or synaptic vesicles. This raised the possibility that other secretory vesicle-associated proteins might be targets of the autoimmune response in type 1 diabetes. To test this hypothesis 56 proteins, two-thirds of which are associated with secretory vesicles, were prepared by in vitro transcription/translation and screened for autoantibodies by liquid phase radioimmunoprecipitation. Two secretory vesicle-associated proteins, VAMP2 and NPY, were identified as new minor autoantigens with 21{\%} and 9{\%}, respectively, of 200 type 1 diabetes sera reacting positively. These findings add support to the hypothesis that secretory vesicle-associated proteins are particularly important, but not the exclusive, targets of the autoimmune response in type 1 diabetes. Selective screening of the human proteome offers a useful approach for identifying new autoantigens in autoimmune diseases.",
keywords = "type 1 diabetes, secretory vesicles, proteome, phosphatase, protein tyrosine, IA-2, GAD65, autoantibodies, autoantigens",
author = "Hiroki Hirai and Junnosuke Miura and Yafang Hu and Helena Larsson and Karin Larsson and {\AA}ke Lernmark and Sten Ivarsson and Tianxia Wu and Albert Kingman and Tzioufas, {Athanasios G} and Notkins, {Abner L}",
year = "2008",
doi = "10.1016/j.clim.2008.01.018",
language = "English",
volume = "127",
pages = "366--374",
journal = "Clinical Immunology",
issn = "1521-6616",
publisher = "Elsevier",
number = "3",

}