Serological Response to M. catarrhalis Outer Membrane Protein MID as Compared to UspA1 and A2.

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title = "Serological Response to M. catarrhalis Outer Membrane Protein MID as Compared to UspA1 and A2.",
abstract = "Morarella catarrhalis immunoglobulin D-binding protein (MID) is a complex antigen with unique immunoglobulin D (IgD)-binding, adhesion, and hernagglutination properties. Previous studies have shown that antibodies raised against MID764-111 in rabbits inhibited M. catarrhalis adhesion to human alveolar epithelial cells, and immunization with MID764-913 resulted in an increased pulmonary clearance in a murine model. Strong immune responses against MID have also consistently been shown in humans. Here, the MID-specified IgG responses were compared to those of ubiquitous surface proteins A1 and A2 (UspA1/A2) using a series of recombinant fragments that spanned all three proteins. Sera were obtained from young children, aged 6 months to 1 year (n = 8) and 2 to 3 years (n = 15), and healthy adults (n = 16). Acute- and convalescent-phase sera from chronic obstructive pulmonary disease (COPD) patients with M. catarrhalis infective exacerbations (n = 23) were also analyzed. Young children, who are at risk of M. catarrhalis infection, had low levels of anti-MID and anti-UspA1/A2 antibodies. Healthy adults and the majority of COPD patients (16/23) had high levels of antibodies directed against, among others, the adhesive domain of MID and the fibronectin- and C3-binding domains of UspA1/A2. Among eight COPD patients in whom a rise in antibody levels could be detected, these functional domains were also the main regions targeted by the antibodies. In addition, human IgG directed against MID was bactericidal and anti-MID antibodies were additive to antibodies targeting UspA1/A2. Hence, the functional domains in these three antigens may have significant potential in a future vaccine against M. catarrhalis.",
author = "Tan, {Thuan Tong} and Christensen, {Jens Jorgen} and Dziegiel, {Morten Hanefeld} and Arne Forsgren and Kristian Riesbeck",
year = "2006",
doi = "10.1128/IAI.00702-06",
language = "English",
volume = "74",
pages = "6377--6386",
journal = "Infection and Immunity",
issn = "1098-5522",
publisher = "American Society for Microbiology",
number = "11",