Serological Response to M. catarrhalis Outer Membrane Protein MID as Compared to UspA1 and A2.

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Serological Response to M. catarrhalis Outer Membrane Protein MID as Compared to UspA1 and A2. / Tan, Thuan Tong; Christensen, Jens Jorgen; Dziegiel, Morten Hanefeld; Forsgren, Arne; Riesbeck, Kristian.

I: Infection and Immunity, Vol. 74, Nr. 11, 2006, s. 6377-6386.

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Tan, Thuan Tong ; Christensen, Jens Jorgen ; Dziegiel, Morten Hanefeld ; Forsgren, Arne ; Riesbeck, Kristian. / Serological Response to M. catarrhalis Outer Membrane Protein MID as Compared to UspA1 and A2. I: Infection and Immunity. 2006 ; Vol. 74, Nr. 11. s. 6377-6386.

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TY - JOUR

T1 - Serological Response to M. catarrhalis Outer Membrane Protein MID as Compared to UspA1 and A2.

AU - Tan, Thuan Tong

AU - Christensen, Jens Jorgen

AU - Dziegiel, Morten Hanefeld

AU - Forsgren, Arne

AU - Riesbeck, Kristian

PY - 2006

Y1 - 2006

N2 - Morarella catarrhalis immunoglobulin D-binding protein (MID) is a complex antigen with unique immunoglobulin D (IgD)-binding, adhesion, and hernagglutination properties. Previous studies have shown that antibodies raised against MID764-111 in rabbits inhibited M. catarrhalis adhesion to human alveolar epithelial cells, and immunization with MID764-913 resulted in an increased pulmonary clearance in a murine model. Strong immune responses against MID have also consistently been shown in humans. Here, the MID-specified IgG responses were compared to those of ubiquitous surface proteins A1 and A2 (UspA1/A2) using a series of recombinant fragments that spanned all three proteins. Sera were obtained from young children, aged 6 months to 1 year (n = 8) and 2 to 3 years (n = 15), and healthy adults (n = 16). Acute- and convalescent-phase sera from chronic obstructive pulmonary disease (COPD) patients with M. catarrhalis infective exacerbations (n = 23) were also analyzed. Young children, who are at risk of M. catarrhalis infection, had low levels of anti-MID and anti-UspA1/A2 antibodies. Healthy adults and the majority of COPD patients (16/23) had high levels of antibodies directed against, among others, the adhesive domain of MID and the fibronectin- and C3-binding domains of UspA1/A2. Among eight COPD patients in whom a rise in antibody levels could be detected, these functional domains were also the main regions targeted by the antibodies. In addition, human IgG directed against MID was bactericidal and anti-MID antibodies were additive to antibodies targeting UspA1/A2. Hence, the functional domains in these three antigens may have significant potential in a future vaccine against M. catarrhalis.

AB - Morarella catarrhalis immunoglobulin D-binding protein (MID) is a complex antigen with unique immunoglobulin D (IgD)-binding, adhesion, and hernagglutination properties. Previous studies have shown that antibodies raised against MID764-111 in rabbits inhibited M. catarrhalis adhesion to human alveolar epithelial cells, and immunization with MID764-913 resulted in an increased pulmonary clearance in a murine model. Strong immune responses against MID have also consistently been shown in humans. Here, the MID-specified IgG responses were compared to those of ubiquitous surface proteins A1 and A2 (UspA1/A2) using a series of recombinant fragments that spanned all three proteins. Sera were obtained from young children, aged 6 months to 1 year (n = 8) and 2 to 3 years (n = 15), and healthy adults (n = 16). Acute- and convalescent-phase sera from chronic obstructive pulmonary disease (COPD) patients with M. catarrhalis infective exacerbations (n = 23) were also analyzed. Young children, who are at risk of M. catarrhalis infection, had low levels of anti-MID and anti-UspA1/A2 antibodies. Healthy adults and the majority of COPD patients (16/23) had high levels of antibodies directed against, among others, the adhesive domain of MID and the fibronectin- and C3-binding domains of UspA1/A2. Among eight COPD patients in whom a rise in antibody levels could be detected, these functional domains were also the main regions targeted by the antibodies. In addition, human IgG directed against MID was bactericidal and anti-MID antibodies were additive to antibodies targeting UspA1/A2. Hence, the functional domains in these three antigens may have significant potential in a future vaccine against M. catarrhalis.

U2 - 10.1128/IAI.00702-06

DO - 10.1128/IAI.00702-06

M3 - Article

VL - 74

SP - 6377

EP - 6386

JO - Infection and Immunity

JF - Infection and Immunity

SN - 1098-5522

IS - 11

ER -