Serum and bronchial lavage fluid concentrations of IL-8, SLPI, sCD14 and sICAM-1 in patients with COPD and asthma

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Serum and bronchial lavage fluid concentrations of IL-8, SLPI, sCD14 and sICAM-1 in patients with COPD and asthma. / Hollander, Camilla; Sitkauskiene, Brigita; Sakalauskas, Raimundas; Westin, Ulla; Janciauskiene, Sabina.

I: Respiratory Medicine, Vol. 101, Nr. 9, 2007, s. 1947-1953.

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Hollander, Camilla ; Sitkauskiene, Brigita ; Sakalauskas, Raimundas ; Westin, Ulla ; Janciauskiene, Sabina. / Serum and bronchial lavage fluid concentrations of IL-8, SLPI, sCD14 and sICAM-1 in patients with COPD and asthma. I: Respiratory Medicine. 2007 ; Vol. 101, Nr. 9. s. 1947-1953.

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TY - JOUR

T1 - Serum and bronchial lavage fluid concentrations of IL-8, SLPI, sCD14 and sICAM-1 in patients with COPD and asthma

AU - Hollander, Camilla

AU - Sitkauskiene, Brigita

AU - Sakalauskas, Raimundas

AU - Westin, Ulla

AU - Janciauskiene, Sabina

PY - 2007

Y1 - 2007

N2 - Background: Airway inflammation is associated with an increased expression and release of inflammatory reactants that regulate processes of cell migration, activation and degranulation. The purpose of this study was to quantify bronchial lavage (BAL) fluid and serum levels of chemokine (IL-8), secretory leukocyte protease inhibitor (SLPI), soluble intracellular adhesion molecules-1 (sICAM-1) and sCD14, as surrogate markers of inflammatory and immune response in asthma and chronic obstructive pulmonary disease (COPD) patients with similar disease duration time. Methods: Biomarkers in serum and BAL fluid from asthma (n = 13) and COPD (n = 25) patients were measured using commercially available ELISA kits. Results: We found that in asthma and COPD groups the concentrations of IL-8 and SLPI are significantly higher in BAL fluid than in serum, while levels of sICAM-1 and sCD14 in BAL fluid are significantly lower than in serum. Of these 4 measured biomarkers, only the BAL IL-8 was higher in COPD patients when compared to asthma (P < 0.05). In both groups, BAL IL-8 correlated with SLPI (r = 0.577, P < 0.01 and r = 0.589, P < 0.05, respectively). In patients with COPD the BAL sICAM-1 correlated with sCD14 (r = 0.576, P < 0.01), while in asthma patients BAL sICAM-1 correlated with FEV,/FVC (r= 0.418, P < 0.01). Moreover, in asthma patients the serum SLPI correlated with sCD14 (r=0.688, P < 0.01) and serum sICAM-1 negatively correlated with FEV1/FVC (r= -0.582, P < 0.05). Conclusion: Our findings point to the importance of selecting a correct biological fluid when analyzing specific biomarkers, and also show that of 4 measured biomarkers, only the BAL IL-8 was higher in COPD patients when compared to asthma. 2007 Published by Elsevier Ltd.

AB - Background: Airway inflammation is associated with an increased expression and release of inflammatory reactants that regulate processes of cell migration, activation and degranulation. The purpose of this study was to quantify bronchial lavage (BAL) fluid and serum levels of chemokine (IL-8), secretory leukocyte protease inhibitor (SLPI), soluble intracellular adhesion molecules-1 (sICAM-1) and sCD14, as surrogate markers of inflammatory and immune response in asthma and chronic obstructive pulmonary disease (COPD) patients with similar disease duration time. Methods: Biomarkers in serum and BAL fluid from asthma (n = 13) and COPD (n = 25) patients were measured using commercially available ELISA kits. Results: We found that in asthma and COPD groups the concentrations of IL-8 and SLPI are significantly higher in BAL fluid than in serum, while levels of sICAM-1 and sCD14 in BAL fluid are significantly lower than in serum. Of these 4 measured biomarkers, only the BAL IL-8 was higher in COPD patients when compared to asthma (P < 0.05). In both groups, BAL IL-8 correlated with SLPI (r = 0.577, P < 0.01 and r = 0.589, P < 0.05, respectively). In patients with COPD the BAL sICAM-1 correlated with sCD14 (r = 0.576, P < 0.01), while in asthma patients BAL sICAM-1 correlated with FEV,/FVC (r= 0.418, P < 0.01). Moreover, in asthma patients the serum SLPI correlated with sCD14 (r=0.688, P < 0.01) and serum sICAM-1 negatively correlated with FEV1/FVC (r= -0.582, P < 0.05). Conclusion: Our findings point to the importance of selecting a correct biological fluid when analyzing specific biomarkers, and also show that of 4 measured biomarkers, only the BAL IL-8 was higher in COPD patients when compared to asthma. 2007 Published by Elsevier Ltd.

KW - bronchial lavage fluid

KW - inflammatory biomarkers

KW - asthma

KW - COPD

KW - serum

U2 - 10.1016/j.rmed.2007.04.010

DO - 10.1016/j.rmed.2007.04.010

M3 - Article

VL - 101

SP - 1947

EP - 1953

JO - Respiratory Medicine

JF - Respiratory Medicine

SN - 1532-3064

IS - 9

ER -