Short-term adaptation improves the fermentation performance of Saccharomyces cerevisiae in the presence of acetic acid at low pH.

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Short-term adaptation improves the fermentation performance of Saccharomyces cerevisiae in the presence of acetic acid at low pH. / Sanchez Nogue, Violeta; Narayanan, Venkatachalam; Gorwa-Grauslund, Marie-Francoise.

I: Applied Microbiology and Biotechnology, Vol. 97, Nr. 16, 2013, s. 7517-7525.

Forskningsoutput: TidskriftsbidragArtikel i vetenskaplig tidskrift

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T1 - Short-term adaptation improves the fermentation performance of Saccharomyces cerevisiae in the presence of acetic acid at low pH.

AU - Sanchez Nogue, Violeta

AU - Narayanan, Venkatachalam

AU - Gorwa-Grauslund, Marie-Francoise

PY - 2013

Y1 - 2013

N2 - The release of acetic acid due to deacetylation of the hemicellulose fraction during the treatment of lignocellulosic biomass contributes to the inhibitory character of the generated hydrolysates. In the present study, we identified a strain-independent adaptation protocol consisting of pre-cultivating the strain at pH 5.0 in the presence of at least 4 g L(-1) acetic acid that enabled aerobic growth and improved fermentation performance of Saccharomyces cerevisiae cells at low pH (3.7) and in the presence of inhibitory levels of acetic acid (6 g L(-1)). During anaerobic cultivation with adapted cells of strain TMB3500, the specific ethanol production rate was increased, reducing the fermentation time to 48 %.

AB - The release of acetic acid due to deacetylation of the hemicellulose fraction during the treatment of lignocellulosic biomass contributes to the inhibitory character of the generated hydrolysates. In the present study, we identified a strain-independent adaptation protocol consisting of pre-cultivating the strain at pH 5.0 in the presence of at least 4 g L(-1) acetic acid that enabled aerobic growth and improved fermentation performance of Saccharomyces cerevisiae cells at low pH (3.7) and in the presence of inhibitory levels of acetic acid (6 g L(-1)). During anaerobic cultivation with adapted cells of strain TMB3500, the specific ethanol production rate was increased, reducing the fermentation time to 48 %.

U2 - 10.1007/s00253-013-5093-5

DO - 10.1007/s00253-013-5093-5

M3 - Article

C2 - 23872959

VL - 97

SP - 7517

EP - 7525

JO - Applied Microbiology and Biotechnology

JF - Applied Microbiology and Biotechnology

SN - 1432-0614

IS - 16

ER -