Single framework recombinant antibody fragments designed for protein chip applications

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Single framework recombinant antibody fragments designed for protein chip applications. / Steinhauer, Cornelia; Wingren, Christer; Malmborg Hager, Ann-Christin; Borrebaeck, Carl.

I: BioTechniques, Nr. Suppl., 2002, s. 38.

Forskningsoutput: TidskriftsbidragArtikel i vetenskaplig tidskrift

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TY - JOUR

T1 - Single framework recombinant antibody fragments designed for protein chip applications

AU - Steinhauer, Cornelia

AU - Wingren, Christer

AU - Malmborg Hager, Ann-Christin

AU - Borrebaeck, Carl

PY - 2002

Y1 - 2002

N2 - High-throughput proteomics, based on the microarray platform, requires stable, highly functional components that will yield a highly sensitive read-out of low, abundance protein. Although antibodies are the best characterized binding molecules for this purpose, only a fraction of them appear to behave satisfactorily in the chip format. Therefore, high demands need to be placed on their molecular design. In the present study, we have focused an recombinant antibody design based on a single framework for protein chip applications, aiming at defining crucial molecular probe parameters. Our results show that engineered human recombinant scFv antibody fragment, that displayed appropriate biophysical properties (molecular [functional] stability in particular) can be generated, making them prime candidates for high-density antibody arrays. In fact a superior framework that displays both multifaceted adsorption properties and very high functional stability over several months on chips (stored in a dried-out state) was identified Taken together designed scFv fragments based on a single molecular scaffold, readily accessible in Large phage display libraries, can undoubtedly meet the requirements of probe content in antibody microarrays, particularly for global proteome analysis.

AB - High-throughput proteomics, based on the microarray platform, requires stable, highly functional components that will yield a highly sensitive read-out of low, abundance protein. Although antibodies are the best characterized binding molecules for this purpose, only a fraction of them appear to behave satisfactorily in the chip format. Therefore, high demands need to be placed on their molecular design. In the present study, we have focused an recombinant antibody design based on a single framework for protein chip applications, aiming at defining crucial molecular probe parameters. Our results show that engineered human recombinant scFv antibody fragment, that displayed appropriate biophysical properties (molecular [functional] stability in particular) can be generated, making them prime candidates for high-density antibody arrays. In fact a superior framework that displays both multifaceted adsorption properties and very high functional stability over several months on chips (stored in a dried-out state) was identified Taken together designed scFv fragments based on a single molecular scaffold, readily accessible in Large phage display libraries, can undoubtedly meet the requirements of probe content in antibody microarrays, particularly for global proteome analysis.

M3 - Article

SP - 38

JO - BioTechniques

T2 - BioTechniques

JF - BioTechniques

SN - 0736-6205

IS - Suppl.

ER -