Single-Cell Analysis Identifies Distinct Stages of Human Endothelial-to-Hematopoietic Transition

Forskningsoutput: TidskriftsbidragArtikel i vetenskaplig tidskrift

Abstract

During development, hematopoietic cells originate from endothelium in a process known as endothelial-to-hematopoietic transition (EHT). To study human EHT, we coupled flow cytometry and single-cell transcriptional analyses of human pluripotent stem cell-derived CD34(+) cells. The resulting transcriptional hierarchy showed a continuum of endothelial and hematopoietic signatures. At the interface of these two signatures, a unique group of cells displayed both an endothelial signature and high levels of key hematopoietic stem cell-associated genes. This interphase group was validated via sort and subculture as an immediate precursor to hematopoietic cells. Differential expression analyses further divided this population into subgroups, which, upon subculture, showed distinct hematopoietic lineage differentiation potentials. We therefore propose that immediate precursors to hematopoietic cells already have their hematopoietic lineage restrictions defined prior to complete downregulation of the endothelial signature. These findings increase our understanding of the processes of de novo hematopoietic cell generation in the human developmental context.

Detaljer

Författare
Enheter & grupper
Externa organisationer
  • University College London
Forskningsområden

Ämnesklassifikation (UKÄ) – OBLIGATORISK

  • Cell- och molekylärbiologi
Originalspråkengelska
Sidor (från-till)10-19
Antal sidor10
TidskriftCell Reports
Volym19
Utgivningsnummer1
StatusPublished - 2017 apr 4
PublikationskategoriForskning
Peer review utfördJa

Relaterad forskningsoutput

Guibentif, C., 2017, Lund: Lund University, Faculty of Medicine. 90 s.

Forskningsoutput: AvhandlingDoktorsavhandling (sammanläggning)

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