SO2-catalyzed steam pretreatment and fermentation of enzymatically hydrolyzed sugarcane bagasse

Forskningsoutput: TidskriftsbidragArtikel i vetenskaplig tidskrift


title = "SO2-catalyzed steam pretreatment and fermentation of enzymatically hydrolyzed sugarcane bagasse",
abstract = "Sugarcane bagasse is a lignocellulosic residue obtained from sugarcane milling, and a potentially interesting raw material that can be used for fuel ethanol production. In the present study, bagasse was steam pretreated at temperatures between 180 and 205 ◦C, with holding times of 5–10 min using SO2 as a catalyst to determine conditions that provide a good recovery of pentoses and a suitable material for enzymatic hydrolysis. Pretreatment conducted at 190 ◦C for 5 min gave a pentose yield of 57{\%}, with only minor amounts of degradation compounds formed. Commercial cellulolytic enzymes were used to hydrolyze the obtained fiber fractions after pretreatment at different water-insoluble solid contents (2{\%}, 5{\%} and 8{\%} WIS). The overall highest sugar yield achieved from bagasse was 87{\%} at 2{\%} WIS. Fermentation tests were made on both the pentose-rich hemicellulose hydrolysate obtained from the pretreatment, and the enzymatic hydrolysates obtained from the fiber fractions using the xylose-fermenting strain of Saccharomyces cerevisiae TMB3400, as well as the natural xylose-utilizing yeast Pichia stipitis CBS 6054. The pretreatment hydrolysates produced at 2{\%} WIS as well as the enzymatic hydrolysates showed a complete glucose fermentability indicating a low toxicity to the yeasts. The best xylose conversion (more than 60{\%}) was achieved by the strain TMB3400 at 2{\%} WIS.",
keywords = "Sugarcane bagasse Bioethanol Xylose Yeast Saccharomyces cerevisiae",
author = "Cristhian Carrasco and Baudel, {Henrique Macedo} and Johan Sendelius and Tobias Modig and Christian Roslander and Mats Galbe and B{\"a}rbel Hahn-H{\"a}gerdal and Guido Zacchi and Gunnar Lid{\'e}n",
year = "2010",
doi = "10.1016/j.enzmictec.2009.10.016",
language = "English",
volume = "46",
pages = "64--73",
journal = "Enzyme and Microbial Technology",
issn = "0141-0229",
publisher = "Elsevier",
number = "2",