SO2-catalyzed steam pretreatment and fermentation of enzymatically hydrolyzed sugarcane bagasse

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SO2-catalyzed steam pretreatment and fermentation of enzymatically hydrolyzed sugarcane bagasse. / Carrasco, Cristhian; Baudel, Henrique Macedo; Sendelius, Johan; Modig, Tobias; Roslander, Christian; Galbe, Mats; Hahn-Hägerdal, Bärbel; Zacchi, Guido; Lidén, Gunnar.

I: Enzyme and Microbial Technology, Vol. 46, Nr. 2, 2010, s. 64-73.

Forskningsoutput: TidskriftsbidragArtikel i vetenskaplig tidskrift

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Carrasco, Cristhian ; Baudel, Henrique Macedo ; Sendelius, Johan ; Modig, Tobias ; Roslander, Christian ; Galbe, Mats ; Hahn-Hägerdal, Bärbel ; Zacchi, Guido ; Lidén, Gunnar. / SO2-catalyzed steam pretreatment and fermentation of enzymatically hydrolyzed sugarcane bagasse. I: Enzyme and Microbial Technology. 2010 ; Vol. 46, Nr. 2. s. 64-73.

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TY - JOUR

T1 - SO2-catalyzed steam pretreatment and fermentation of enzymatically hydrolyzed sugarcane bagasse

AU - Carrasco, Cristhian

AU - Baudel, Henrique Macedo

AU - Sendelius, Johan

AU - Modig, Tobias

AU - Roslander, Christian

AU - Galbe, Mats

AU - Hahn-Hägerdal, Bärbel

AU - Zacchi, Guido

AU - Lidén, Gunnar

PY - 2010

Y1 - 2010

N2 - Sugarcane bagasse is a lignocellulosic residue obtained from sugarcane milling, and a potentially interesting raw material that can be used for fuel ethanol production. In the present study, bagasse was steam pretreated at temperatures between 180 and 205 ◦C, with holding times of 5–10 min using SO2 as a catalyst to determine conditions that provide a good recovery of pentoses and a suitable material for enzymatic hydrolysis. Pretreatment conducted at 190 ◦C for 5 min gave a pentose yield of 57%, with only minor amounts of degradation compounds formed. Commercial cellulolytic enzymes were used to hydrolyze the obtained fiber fractions after pretreatment at different water-insoluble solid contents (2%, 5% and 8% WIS). The overall highest sugar yield achieved from bagasse was 87% at 2% WIS. Fermentation tests were made on both the pentose-rich hemicellulose hydrolysate obtained from the pretreatment, and the enzymatic hydrolysates obtained from the fiber fractions using the xylose-fermenting strain of Saccharomyces cerevisiae TMB3400, as well as the natural xylose-utilizing yeast Pichia stipitis CBS 6054. The pretreatment hydrolysates produced at 2% WIS as well as the enzymatic hydrolysates showed a complete glucose fermentability indicating a low toxicity to the yeasts. The best xylose conversion (more than 60%) was achieved by the strain TMB3400 at 2% WIS.

AB - Sugarcane bagasse is a lignocellulosic residue obtained from sugarcane milling, and a potentially interesting raw material that can be used for fuel ethanol production. In the present study, bagasse was steam pretreated at temperatures between 180 and 205 ◦C, with holding times of 5–10 min using SO2 as a catalyst to determine conditions that provide a good recovery of pentoses and a suitable material for enzymatic hydrolysis. Pretreatment conducted at 190 ◦C for 5 min gave a pentose yield of 57%, with only minor amounts of degradation compounds formed. Commercial cellulolytic enzymes were used to hydrolyze the obtained fiber fractions after pretreatment at different water-insoluble solid contents (2%, 5% and 8% WIS). The overall highest sugar yield achieved from bagasse was 87% at 2% WIS. Fermentation tests were made on both the pentose-rich hemicellulose hydrolysate obtained from the pretreatment, and the enzymatic hydrolysates obtained from the fiber fractions using the xylose-fermenting strain of Saccharomyces cerevisiae TMB3400, as well as the natural xylose-utilizing yeast Pichia stipitis CBS 6054. The pretreatment hydrolysates produced at 2% WIS as well as the enzymatic hydrolysates showed a complete glucose fermentability indicating a low toxicity to the yeasts. The best xylose conversion (more than 60%) was achieved by the strain TMB3400 at 2% WIS.

KW - Sugarcane bagasse Bioethanol Xylose Yeast Saccharomyces cerevisiae

U2 - 10.1016/j.enzmictec.2009.10.016

DO - 10.1016/j.enzmictec.2009.10.016

M3 - Article

VL - 46

SP - 64

EP - 73

JO - Enzyme and Microbial Technology

T2 - Enzyme and Microbial Technology

JF - Enzyme and Microbial Technology

SN - 0141-0229

IS - 2

ER -