Solid-liquid interface method (SLIM): a new crystallization method for proteins

Forskningsoutput: TidskriftsbidragArtikel i vetenskaplig tidskrift

Abstract

Despite impressive advances in theories, methods and technologies, crystallization still remains a serious bottleneck in structural determination of macromolecules. Here we present a novel solid-liquid interface method (SLIM) for protein crystallization, based on the pre-adding and drying of a crystallization reagent, and thereafter the dispensing of a protein solution to the dried media to initiate crystallization from the solid-liquid interface. Not only quick and easy to perform, the method also allows for a less concentrated protein solution for setting up crystallization trials.

Detaljer

Författare
  • Erik Brostromer
  • Jie Nan
  • Lan-Fen Li
  • Xiao-Dong Su
Enheter & grupper
Externa organisationer
  • Peking University
Forskningsområden

Nyckelord

Originalspråkengelska
Sidor (från-till)634-8
Antal sidor5
TidskriftBiochemical and Biophysical Research Communications
Volym386
Utgivningsnummer4
StatusPublished - 2009 sep 4
PublikationskategoriForskning
Peer review utfördJa