Spermatogonia Loss Correlates with LAMA 1 Expression in Human Prepubertal Testes Stored for Fertility Preservation

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Spermatogonia Loss Correlates with LAMA 1 Expression in Human Prepubertal Testes Stored for Fertility Preservation. / Kurek, Magdalena; Åkesson, Elisabet; Yoshihara, Masahito; Oliver, Elizabeth; Cui, Yanhua; Becker, Martin; Alves-Lopes, João Pedro; Bjarnason, Ragnar; Romerius, Patrik; Sundin, Mikael; Norén Nyström, Ulrika; Langenskiöld, Cecilia; Vogt, Hartmut; Henningsohn, Lars; Petersen, Cecilia; Söder, Olle; Guo, Jingtao; Mitchell, Rod T.; Jahnukainen, Kirsi; Stukenborg, Jan Bernd.

I: Cells, Vol. 10, Nr. 2, 241, 2021.

Forskningsoutput: TidskriftsbidragArtikel i vetenskaplig tidskrift

Harvard

Kurek, M, Åkesson, E, Yoshihara, M, Oliver, E, Cui, Y, Becker, M, Alves-Lopes, JP, Bjarnason, R, Romerius, P, Sundin, M, Norén Nyström, U, Langenskiöld, C, Vogt, H, Henningsohn, L, Petersen, C, Söder, O, Guo, J, Mitchell, RT, Jahnukainen, K & Stukenborg, JB 2021, 'Spermatogonia Loss Correlates with LAMA 1 Expression in Human Prepubertal Testes Stored for Fertility Preservation', Cells, vol. 10, nr. 2, 241. https://doi.org/10.3390/cells10020241

APA

Kurek, M., Åkesson, E., Yoshihara, M., Oliver, E., Cui, Y., Becker, M., Alves-Lopes, J. P., Bjarnason, R., Romerius, P., Sundin, M., Norén Nyström, U., Langenskiöld, C., Vogt, H., Henningsohn, L., Petersen, C., Söder, O., Guo, J., Mitchell, R. T., Jahnukainen, K., & Stukenborg, J. B. (2021). Spermatogonia Loss Correlates with LAMA 1 Expression in Human Prepubertal Testes Stored for Fertility Preservation. Cells, 10(2), [241]. https://doi.org/10.3390/cells10020241

CBE

Kurek M, Åkesson E, Yoshihara M, Oliver E, Cui Y, Becker M, Alves-Lopes JP, Bjarnason R, Romerius P, Sundin M, Norén Nyström U, Langenskiöld C, Vogt H, Henningsohn L, Petersen C, Söder O, Guo J, Mitchell RT, Jahnukainen K, Stukenborg JB. 2021. Spermatogonia Loss Correlates with LAMA 1 Expression in Human Prepubertal Testes Stored for Fertility Preservation. Cells. 10(2):Article 241. https://doi.org/10.3390/cells10020241

MLA

Vancouver

Author

Kurek, Magdalena ; Åkesson, Elisabet ; Yoshihara, Masahito ; Oliver, Elizabeth ; Cui, Yanhua ; Becker, Martin ; Alves-Lopes, João Pedro ; Bjarnason, Ragnar ; Romerius, Patrik ; Sundin, Mikael ; Norén Nyström, Ulrika ; Langenskiöld, Cecilia ; Vogt, Hartmut ; Henningsohn, Lars ; Petersen, Cecilia ; Söder, Olle ; Guo, Jingtao ; Mitchell, Rod T. ; Jahnukainen, Kirsi ; Stukenborg, Jan Bernd. / Spermatogonia Loss Correlates with LAMA 1 Expression in Human Prepubertal Testes Stored for Fertility Preservation. I: Cells. 2021 ; Vol. 10, Nr. 2.

RIS

TY - JOUR

T1 - Spermatogonia Loss Correlates with LAMA 1 Expression in Human Prepubertal Testes Stored for Fertility Preservation

AU - Kurek, Magdalena

AU - Åkesson, Elisabet

AU - Yoshihara, Masahito

AU - Oliver, Elizabeth

AU - Cui, Yanhua

AU - Becker, Martin

AU - Alves-Lopes, João Pedro

AU - Bjarnason, Ragnar

AU - Romerius, Patrik

AU - Sundin, Mikael

AU - Norén Nyström, Ulrika

AU - Langenskiöld, Cecilia

AU - Vogt, Hartmut

AU - Henningsohn, Lars

AU - Petersen, Cecilia

AU - Söder, Olle

AU - Guo, Jingtao

AU - Mitchell, Rod T.

AU - Jahnukainen, Kirsi

AU - Stukenborg, Jan Bernd

PY - 2021

Y1 - 2021

N2 - Fertility preservation for male childhood cancer survivors not yet capable of producing mature spermatozoa, relies on experimental approaches such as testicular explant culture. Although the first steps in somatic maturation can be observed in human testicular explant cultures, germ cell depletion is a common obstacle. Hence, understanding the spermatogonial stem cell (SSC) niche environment and in particular, specific components such as the seminiferous basement membrane (BM) will allow progression of testicular explant cultures. Here, we revealed that the seminiferous BM is established from 6 weeks post conception with the expression of laminin alpha 1 (LAMA 1) and type IV collagen, which persist as key components throughout development. With prepubertal testicular explant culture we found that seminiferous LAMA 1 expression is disrupted and depleted with culture time correlating with germ cell loss. These findings highlight the importance of LAMA 1 for the human SSC niche and its sensitivity to culture conditions.

AB - Fertility preservation for male childhood cancer survivors not yet capable of producing mature spermatozoa, relies on experimental approaches such as testicular explant culture. Although the first steps in somatic maturation can be observed in human testicular explant cultures, germ cell depletion is a common obstacle. Hence, understanding the spermatogonial stem cell (SSC) niche environment and in particular, specific components such as the seminiferous basement membrane (BM) will allow progression of testicular explant cultures. Here, we revealed that the seminiferous BM is established from 6 weeks post conception with the expression of laminin alpha 1 (LAMA 1) and type IV collagen, which persist as key components throughout development. With prepubertal testicular explant culture we found that seminiferous LAMA 1 expression is disrupted and depleted with culture time correlating with germ cell loss. These findings highlight the importance of LAMA 1 for the human SSC niche and its sensitivity to culture conditions.

KW - basal membrane

KW - germ cells

KW - infertility

KW - late effects

KW - seminiferous tubules

KW - Sertoli cells

KW - spermatogonia

KW - stem cell niche

U2 - 10.3390/cells10020241

DO - 10.3390/cells10020241

M3 - Article

C2 - 33513766

AN - SCOPUS:85100612669

VL - 10

JO - Cells

JF - Cells

SN - 2073-4409

IS - 2

M1 - 241

ER -