Stable lodinated polypeptide hormones prepared by polyacrylamide gel electrophoresis

Forskningsoutput: TidskriftsbidragArtikel i vetenskaplig tidskrift

Abstract

Iodination of several insulin and proinsulin preparations, human growth hormone and bovine pancreatic polypeptide was performed using H2O2 and lactoperoxidase or chloramine T. The iodination mixtures were fractionated by polyacrylamide gel electrophoresis at pH 9.15 in long gel rods followed by simple elution of the iodinated products from thin gel slices. With this method 125I tracers with long shelf life and high specific activity suitable for radioimmunoassays could easily be obtained.

Detaljer

Författare
Externa organisationer
  • Hagedorn Research Institute
Forskningsområden

Ämnesklassifikation (UKÄ) – OBLIGATORISK

  • Endokrinologi och diabetes
Originalspråkengelska
Sidor (från-till)165-176
Antal sidor12
TidskriftAnalytical Biochemistry
Volym107
Utgåva nummer1
StatusPublished - 1980 jan 1
PublikationskategoriForskning
Peer review utfördJa
Externt publiceradJa