Standardization of Cystatin C: development of primary and secondary reference preparations.

Forskningsoutput: TidskriftsbidragArtikel i vetenskaplig tidskrift

Abstract

A Primary Reference Preparation has been produced using pure, recombinant, Cystatin C in a solvent of 0.1 mol/L KCl. Dry mass determination of the Primary Reference Preparation resulted in a Cystatin C concentration of 5.20 g/L. Agarose-electrophoresis and SDS-electrophoresis, as well as N-terminal sequencing, verified the purity, homogeneity and identity of Cystatin C in the Primary Reference Preparation. For the Secondary Reference Preparation, a serum pool was collected and stabilized. A pilot batch was made to verify the selected procedure and spiking with the pure, recombinant Cystatin C. The final Secondary Reference Preparation is now produced (4468 vials) and ready for value assignment and further characterization.

Detaljer

Författare
Enheter & grupper
Forskningsområden

Ämnesklassifikation (UKÄ) – OBLIGATORISK

  • Läkemedelskemi
  • Farmakologi och toxikologi

Nyckelord

Originalspråkengelska
Sidor (från-till)67-70
TidskriftScandinavian Journal of Clinical & Laboratory Investigation
Volym68
Utgivningsnummers241
StatusPublished - 2008
PublikationskategoriForskning
Peer review utfördJa

Relaterad forskningsoutput

Veronica Lindström, 2013, Division of Clinical Chemistry and Pharmacology, Faculty of Medicine, Lund University. 62 s.

Forskningsoutput: AvhandlingDoktorsavhandling (sammanläggning)

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