Stimulation and inhibition of insulin release by an amino-reactive probe of plasma membrane

Forskningsoutput: TidskriftsbidragArtikel i vetenskaplig tidskrift

Abstract

4-Acetamido-4′-isothiocyanostilbene-2,2′-disulphonic acid (SITS), an amino-reacting probe of plasma membranes, stimulated the release of insulin from micro-dissected pancreatic islets of ob/ob-mice. This effect of SITS was inhibited by adrenaline or by calcium deficiency. SITS did not inhibit the insulin-releasing action of glucose or leucine but rather potentiated the effect of glucose. In contrast, SITS markedly depressed the insulin secretory response to chloromercuribenzene-p-sulphonic acid. It is suggested that by reacting with the plasma membranes SITS may induce secretagogic ionic fluxes in the β-cells. In addition, SITS apparently inhibits the secretagogic recognition of chloromercuribenzene-p-sulphonic acid, presumably by preventing the organic mercurial from reacting with certain membrane thiol groups.

Detaljer

Författare
  • Bo Hellman
  • Lars Åke Idahl
  • Åke Lernmark
  • Janove Sehlin
  • Inge Bert Täljedal
Externa organisationer
  • Umeå University
Originalspråkengelska
Sidor (från-till)135-142
TidskriftThe Journal of Membrane Biology
Volym14
Utgåva nummer1
StatusPublished - 1973 dec 1
PublikationskategoriForskning
Peer review utfördJa
Externt publiceradJa